2023年获第八届中国创新挑战赛生物制造技术专题赛二等奖

代表性论文：

1. Shi, T., Chen, X.-M., and Zhang, Y.-H.P.J., New technologies and industrialization progress in healthy sugar biomanufacturing based on in vitro synthetic biology. Synthetic Biology Journal, 2025. 6: 1-17.

2. Fan, L., Shi, T., Chen, X., Li, Y., Han, P., Jensen, P.R., and Zhang, Y.-H.P.J., Biosynthesis of a healthy natural sugar D-tagatose: advances and opportunities. Critical Reviews in Biotechnology, 2025: 1-16.(co-first author)

3. Shi, T., Song, Z., Song, S.-Y., and Zhang, Y.-H.P.J., In vitro BioTransformation (ivBT): New frontier of industrial biomanufacturing. Synthetic Biology Journal, 2024. 5(6): 1-24.

4. Han, P., Wang, X., Li, Y., Wu, H., Shi, T.*, and Shi, J., Synthesis of a healthy sweetener D-tagatose from starch catalyzed by semiartificial cell factories. Journal of Agricultural and Food Chemistry, 2023. 71(8): 3813-3820.

5. Ye, J., Li, Y.J., Bai, Y.Q., Zhang, T., Jiang, W., Shi, T.*, Wu, Z.J., and Zhang, Y.-H.P.J., A facile and robust T7-promoter-based high-expression of heterologous proteins in Bacillus subtilis. Bioresources and Bioprocessing, 2022. 9(56): 1-12.

6. Li, Y., Shi, T., Han, P., and You, C., Thermodynamics-driven production of value-added D-allulose from inexpensive starch by an in vitro enzymatic synthetic biosystem. ACS Catalysis, 2021. 11(9): 5088-5099.(Co-first author)

7. Li, Y., Han, P., Wang, J., Shi, T.*, and You, C., Production of myo-inositol: Recent advance and prospective.      Biotechnology and Applied Biochemistry, 2021. 69(3): 1101-1111.

8. Liu, S., Wang, J., Zhu, Z., Shi, T.*, and Job Zhang, Y.-H.P.J., Efficient secretory production of large-size heterologous enzymes in Bacillus subtilis: a secretory partner and directed evolution. Biotechnology and Bioengineering, 2020. 117(10): 2957-2968.

9. Shi, T., Liu, S., and Zhang, Y.-H.P.J., CO₂ fixation for malate synthesis energized by starch via in vitro metabolic engineering. Metabolic Engineering, 2019. 55: 152-160.

10. Wang, G., Shi, T., Chen, T., Wang, X., Wang, Y., Liu, D., Guo, J., Fu, J., Feng, L., Wang, Z., and Zhao, X., Integrated whole-genome and transcriptome sequence analysis reveals the genetic characteristics of a riboflavin-overproducing Bacillus subtilis. Metabolic Engineering, 2018. 48: 138-149.(Co-first author)

11. Shi, T., Han, P., You, C., and Zhang, Y.-H.P.J., An in vitro synthetic biology platform for emerging industrial biomanufacturing: Bottom-up pathway design. Synthetic and Systems Biotechnology, 2018. 3(3): 186-195.

12. Shi, T., Wang, Y., Wang, Z., Wang, G., Liu, D., Fu, J., Chen, T., and Zhao, X.M., Deregulation of purine pathway in Bacillus subtilis and its use in riboflavin biosynthesis. Microbial Cell Factories, 2014. 13(1): 101.

13. Shi, T., Wang, G.L., Wang, Z.W., Fu, J., Chen, T., and Zhao, X.M., Establishment of a markerless mutation delivery system in Bacillus subtilis stimulated by a double-strand break in the chromosome. PloS One, 2013. 8(11).

代表性专利：

1. 张以恒、石婷、刘博华，一种海藻糖6-磷酸的体外生物转化合成体系及其应用，ZL202511134795.X

2. 马延和、石婷、宋云洪、李运杰、张婷，重组微生物、其制备方法及在生产塔格糖中的应用，ZL202011224203.0

3. 马延和、石婷、李运杰、韩平平、李元，产塔格糖的枯草芽孢杆菌基因工程菌及制备塔格糖的方法，ZL202110006046.4

4. 马延和、石婷、韩平平、李运杰，人工油体固定化多酶生产塔格糖的方法，ZL202110370762.0

5. 马延和、石婷、韩平平，仿生硅矿化微囊固定化多酶生产塔格糖的方法，ZL202110370763.5

6. 马延和、石婷、韩平平，一种固定化多酶体系生产塔格糖的方法，ZL202110330594.2

7. 马延和、石婷、韩平平，一种用于塔格糖生产的固定化细胞的制备方法及其应用，ZL202110757673.1

8. 马延和、石婷、韩平平，一种用于甘露糖生产的固定化细胞的制备方法，ZL202110757694.3

9. 马延和、石婷、李运杰，用于高效外源蛋白表达和高密度培养的工程枯草芽孢杆菌，ZL202110208427.0

10. 马延和、石婷、李元，一种制备高纯度结晶塔格糖的方法，ZL202110223481.2

11. 游淳、石婷、刘珊，高效分泌异淀粉酶的基因工程菌，ZL201911282988.4

12. 游淳、石婷、刘珊，用于外源蛋白分泌的糖苷水解酶融合表达系统，ZL201911282970.4

13. 游淳、石婷，一种枯草芽孢杆菌全细胞催化淀粉制备肌醇的方法，ZL201911282987.X

14. 游淳、李运杰、石婷，一种酶促合成阿洛酮糖的方法，ZL202110106706.6