报告题目：Engineering Dynamic Cellular Metabolism for Microbial Manufacturing

　　报 告 人：闫亚军 佐治亚大学教授

　　报告时间：3月9 日（周五） 9:30-11:00

　　报告地点：天津工业生物所C408会议室

　　报告摘要：Achieving viable productivities, titers and yields is extremely critical to microbial production. However, cellular and environmental changes may greatly impair microbial production due to that the producing hosts are incapable of responding to the changes dynamically. Thus, it is highly desired to develop dynamic control techniques to engineer cells with great biological robustness, which can automatically adjust their metabolic activities to the changing conditions to realize optimal production efficiency. In this presentation, we describe a general strategy for developing regulatory networks to implement dynamic controls of cellular metabolism for production improvement in microbial hosts. The regulatory elements consist of promoter, sensor-regulator, and antisense RNA. The dynamic regulation can occur at both transcriptional and post-transcriptional levels and involve not only heterologous genes but also host native metabolism. More importantly, this dynamic regulation can execute orthogonal and simultaneous “up-regulation” and “down-regulation” functions through using a single promoter-regulator system in response to the same inducing molecule, As a proof-of-concept, we utilize the muconic acid biosynthetic pathway developed in our group to examine the efficiency of this strategy on muconic acid production in Escherichia coli. Briefly, the research work involves the development and characterization of hybrid promoter-regulator system and dynamic regulatory network, and examination of the efficiency of dynamic regulatory network on muconic acid production.

　　报告人简介：闫亚军教授，分别于1999年、2002年获得北京化工大学生化工程学士学位和硕士学位；2008年，在纽约州立大学布法罗分校化学和生物工程系获得博士学位，研究方向为代谢工程；2008年至2010年，在加利福尼亚大学洛杉矶分校进行博士后研究，代谢工程和合成生物学为其主要研究领域。闫博士于2010年加入佐治亚大学，担任生物化学工程学助理教授，并于2015年晋升为终身职位副教授。闫博士目前的研究兴趣包括微生物代谢工程和合成生物学，生产重要药物分子、可再生燃料以及化学品。