报告题目：Production of human hemoglobin in yeast
报告摘要：As we are being more numerous, live longer and develop new procedures to cure or alleviate diseases and extend life span, there is a growing need for blood substitues, particularly oxygen carriers. Hemoglobin is a common molecule used for oxygen transfer in many vertebrates, so one can use blood transfusions from other humans or isolate hemoglobin from expired transfusions or animals. The problem with transfusions is that the supply is limited, storage complicated (has to be refrigirated and expires relativly quickly) and there is continous risk of transering untested diseases.
We are interested in producing human globin proteins such as neuroglobin, myoglobin and hemoglobin and study their effects on cells, but particulary we are interesed in producing human adult (HbA) and fetal (HbF) hemoglobins with the scope of developing efficient cell factories that could be used as a sustainable and safe source of human hemoglobin for medical applications. Hemoglobin is a tetramer composed of two alpha and two beta or gamma chains (in HbA or HbF, respectivly) and 4 heme prosthetic groups, which coordinate the iron atom which is responsible for oxygen binding, therefore besides optimizing the production of the peptide chains, we also need to consider optimization of the heme production and iron incorporation. All these aspects will be addressed during the talk.
报告人简介：Dina Petranovic has a Msc (1994-1999) in Molecular Biology from Zagreb University in Croatia, PhD (2000-2004) in Molecular Microbiology from University Paris XI in France, two postdocs at Technical University in Denmark (2004-2006, in Microbial Physiology and Genetics, and 2006-2008 in Microbial Biotechnology), and assistant professorship (2008-2012), docent (2012) and tenure as associate professor (since 2013) at Chalmers University of Technology in Sweden.
During her career Dina has also been visiting scientist in Rich Losick's lab at Harvard in 2006 and visiting professor in Susan Lindquist's Lab at MIT in 2012.
During her PhD and postdocs Dina worked with Gram+ bacteria Lactococcus lactis and Bacillus subtilis where she studied transcriptional regulation via the pleiotropic regulator CodY, signal transductions via the phosphorelay PTS-system and protein tyrosin phosphorylation.
Dina is CEO of a non-profit organization Molecular Frontiers, which is under the Royal Scwedish Academy of Science, editor of FEMS Yeast Research, co-director in the European Federation of Biotechnology and member of several societies (IMES, SBE etc). Dina is also a teacher, supervisor and examiner at Chalmers University in Göteborg and mentor at Karolinska Institute in Stockholm, and was awarded the pedagodical prize at Chalmers in 2013.