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【4月28日报告】Predictive scale up using micro and mini bioreactors
  发布时间:2016-04-25    供稿部门:

  报告题目Predictive scale up using micro and mini bioreactors

  报 告 人牟敦刚 Moubio Knowledge co.  

  报告时间428日(周四) 10:00-11:00   

  报告地点:天津工业生物所C408报告厅   

  报告人简介麻省理工学院(MIT)生化工程博士。1986年底受聘于刚刚成立的台湾生物技术开发中心(DCB),具有40多年生物技术相关领域的工作经验,包括生物技术研究与开发、发酵与制造、研究生和本科生的教学、科研管理支持、政府科技项目咨询、市场和行业信息服务、工业和商业培训研讨会、生物技术企业的发展、工业联络员、构建政府-产业-大学网络等。 

  客座全职/兼职讲师、工业培训、咨询、微型生物反应器的创新。自2001Moubio建立以来一直通过研讨会传授发酵技能,同时是台北国立图书馆的英语辅导志愿者。

    两部生物技术中文商业著作;台湾与国际性质在内专利超过10个;发表刊物超过50篇,包括技术期刊、会议海报与介绍;以主席、组织者、演讲者和代表等身份参与了众多国际生物技术研讨会、例会、商务与贸易会议;作为美国化学学会和工业微生物与生物技术学会成员已有30年。

    报告摘要One well known drawback of shake flask (often conical Erlenmeyer flasks) culture is its limited surface aeration and atmospheric gas exchange. Ultimate enhancement of oxygen transfer in shaker culture relies on atmospheric oxygen enrichment or culture volume reduction or both. They soon made high throughput studies of micro-bioreactors cultures a success as soon as the micro-titer-plates and its readers became available. Like shake flask, their shortcoming in scale up remains with the unrealistic surface aeration, and cannot be resolved simply by a matching kLa – mass transfer coefficient kL, multiplied by total gas-liquid interfacial area, a. On the other hand, impeller dispersed line gas sparging in larger scale stirred tank reactor (STR) is not easily scale down to or reproduced in mini and micro scale vessels like shake flask or micro titer plate without compromising their advantage in simplicity and economy. As success of combinatorial DNA reconstruction and its subsequent high volume phenotype screen and validation spreads in recent decade, predictive scale up, like realistic up-scale stirring, gas-liquid mixing and gas phase oxygen and carbon dioxide partial pressures, in mini or micro scale reaction vessels has driven the high throughput fermentation screening device market ever since. This presentation will go over pros and cons of recent mini jar (in 10-100s mL) and micro well (in 100s µL) bioreactors in gas-liquid mixing design, and how bioreactor innovations can benefit high throughput process validation and predictive scale up in everyday microbiology laboratory. Keywords: oxygen transfer coefficient, shake flask, micro well bioreactor, mini jar fermentor, fermentation scale up.
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