报告题目：Development of an Automated Synthetic Biology Foundry
报 告 人：晁然博士 （伊利诺伊大学厄巴纳-香槟分校）
报告地点：天津工业生物所 C408 学术报告厅
报告摘要：Synthetic biology has great promises in health, biochemical industry, as well as fundamental research. Synthetic biologists engineer biological systems design-build-test cycles, however, constrained by the high labor cost, low throughput, and artifacts in this learning cycle. We have recently established Illinois Biological Foundry for Advanced Biomanufacturing (iBioFAB), which consists of a computational framework and an integrated robotic system. The computational framework includes a scheduler that orchestrates all automation events. The robotic system features a centralized robotic arm that transfers samples among more than 20 instruments and is capable of conducting most common experiments in synthetic biology. To demonstrate its unprecedented power, we have implemented a TALEN synthesis process on iBioFAB and able to generate up to 800 transcription activation-like effector nucleases (TALENs) per day with minimal human intervention. In addition, the iBioFAB has been used to automate the entire process of the RNA interference RNAi-assisted genome evolution (RAGE) process we recently developed for reprogramming of Saccharomyces cerevisiae. Through iterative cycles of RNAi induced reduction-of-function library creation coupled with high throughput screening, acetate toleration of the yeast strain has been significantly improved by accumulating beneficial genetic modifications.
报告人简介：晁然本科曾就读于浙江大学，后获加拿大英属哥伦比亚大学化工与生物工程学士学位。现攻读伊利诺伊大学香槟分校化学与分子生物工程博士，师从赵惠民教授，在卡尔·乌斯基因组学研究所生物系统设计实验室从事合成生物学自动化研究。晁然负责建造了世界首台全自动合成生物学柔性加工平台Illinois Biological Foundry for Advanced Biomanufacturing (iBioFAB)，可自动完成大多数分子及细胞操作。该平台已被应用于高通量DNA组装以及基因组修改，验证了其灵活性及强大的生物加工能力。