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姓名 |
孙际宾 |
性别 |
男 |
专家类别 |
N/A |
职称 |
研究员 |
学历 |
博士研究生 |
电话 |
022-84861949 |
传真 |
N/A |
电子邮件 |
sunjibin@tib.cas.cn |
地址 |
天津空港经济区西七道32号 |
邮编 |
300308 |
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简历 |
1989.09-1993.09 山东大学微生物系微生物工程专业,学士
1993.09-1996.07 山东大学微生物系/华东理工大学生化工程系,硕士
1996.07-2000.09 山东大学微生物系微生物技术国家重点实验室,助教/讲师
2000.10-2002.10 DAAD德意志学术交流奖学金资助到德国国家生物工程中心GBF学习
2001.10-2004.10 德国布伦瑞克工业大学/ GBF 博士
2004.10-2008.03 德国亥姆霍兹传染病研究中心(原GBF)系统生物学课题组,博士后
2008.03-至今 中国科学院天津工业生物技术研究所,研究员
2008.05-2010.01 兼任汉堡工业大学生物系统与过程工程研究所科学家,工业生物技术领域协调人
2011.05-2015.05 中国科学院系统微生物工程重点实验室,副主任
2012.10-至今 中国科学院天津工业生物技术研究所,副所长
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研究方向: |
定位于工业微生物系统生物学。致力于发展工业微生物的先进研究方法,认知工业微生物的高产抗逆分子基础,设计和创造符合工业化需求的微生物。主要研究方向:1、工业微生物的系统生物学;2、氨基酸工业菌株改造与创制。
发表SCI论文60余篇,申请中国发明专利40余项,已授权12项,PCT专利3项,美日等国授权5项。
代表论著:
1. Dele-Osibanjo T#, Li Q #, Zhang X #, Guo X, Feng J, Liu J, Sun X, Wang X, Zhou W, Zheng P*, Sun J*, Ma Y. Growth-coupled evolution of phosphoketolase to improve l-glutamate production by Corynebacterium glutamicum. Appl Microb Biotech. 2019
2. Wang W#, Li Q#, Sun C, Cai Z, Zheng X, Guo X, Ni X, Zhou W, Guo Y, Zheng P*, Chen C, Sun J* , Li Y, Ma Y. GREACE-assisted adaptive laboratory evolution in endpoint fermentation broth enhances lysine production by Escherichia coli. Microb Cell Fact. 2019
3. Cairns TC#, Feurstein C#, Zheng X, Zheng P, Sun J*, Meyer V*. A quantitative image analysis pipeline for the characterization of filamentous fungal morphologies as a tool to uncover targets for morphology engineering: a case study using aplD in Aspergillus niger. Biotech Biofuels. 2019
4. Zhu C#, Chen J#, Wang Y, Wang L, Guo X, Chen N, Zheng P*, Sun J*, Ma Y. Enhancing 5‐aminolevulinic acid tolerance and production by engineering the antioxidant defense system of Escherichia coli. Biotechnol Bioeng. 2019
5. Zhao J#, Liu C#, Guo X, Wang J, Liu H, Zheng P*, Sun J*, Ma Y. Efficient production of trans-3-hydroxyproline by a bacterial trans-3-proline hydroxylase and characterization of enzymatic properties. Biochem Eng J, 2019, 147: 57-61.
6. S Sun D#, Chen J#, Wang Y, Li M, Rao D, Guo Y, Chen N, Zheng P*, Sun J*, Ma Y. Metabolic Engineering of Corynebacterium glutamicum by Synthetic Small Regulatory RNAs. J Industr Microb & Biotech, 2019, 46 (2): 203–208.
7. Liu J#, Wang Y #, Lu Y, Ni X, Guo X, Zhao J, Chen J, Dele-Osibanjo T, Zheng P*, Sun J*, Ma Y. Mutations on Peptidoglycan Synthesis Gene ponA Improve Electro-transformation Efficiency of Corynebacterium glutamicum ATCC 13869. Appl Envir Microb, 2019, 84(24): e02225-18
8. Zheng X, Yu J, Cairns TC, Zhang L, Zhang Z, Zhang Q, Zheng P*, Sun J*, Ma Y. Comprehensive Improvement of Sample Preparation Methodologies Facilitates Dynamic Metabolomics of Aspergillus niger. Biotech J, 2019, 14(3): 1800315
9. Wang X#, Liu J#, Zhao J, Ni X, Zheng P, Guo X, Sun C, Sun J*, Ma Y. Efficient production of trans-4-hydroxy-L-proline from glucose using a new trans-proline 4-hydroxylase in Escherichia coli. J Biosci Bioeng, 2018, 126 (4): 470-477.
10. Liu C#, Zhao J#, Liu J, Guo X, Rao D, Liu H, Zheng P*, Sun J*, Ma Y. Simultaneously improving the activity and thermostability of a new proline 4-hydroxylase by loop grafting and site-directed mutagenesis. Appl Microb Biotech, 2018, 103 (10): 265–277.
11. Tuyishime P#, Wang Y#, Fan L, Zhang Q, Li Q, Zheng P*, Sun J*, Ma Y. Engineering Corynebacterium glutamicum for methanol-dependent growth and glutamate production. Metab Eng, 2018, 49:20-231.
12. Fan L#, Wang Y#, Tuyishime P, Gao N, Li Q, Zheng P*, Sun J*, Ma Y. Engineering Artificial Fusion Proteins for Enhanced Methanol Bioconversion. ChemBioChem, 2018, 19 (23): 2465-2471.
13. Zhang Q#, Zheng X#, Wang Y, Yu J, Zhang Z, Dele-Osibanjo T, Zheng P*, Sun J*, Jia S, Ma Y. Comprehensive Optimization of the Metabolomics Methodology for Metabolite Profiling of Corynebacterium glutamicum. Appl Microb Biotech, 2018, 102 (16): 7113-7121.
14. Zheng X, Zheng P, Zhang K, Cairns TC, Meyer V, Sun J*, Ma Y. 5S rRNA Promoter for Guide RNA Expression Enabled Highly Efficient CRISPR/Cas9 Genome Editing in Aspergillus niger. ACS Synth Biol., 2018, e7b00456.
15. Wang Y#, Liu Y #, Liu J, Guo Y, Fan L, Ni X, Zheng X, Wang M*, Zheng P*, Sun J*, Ma Y. MACBETH: Multiplex automated Corynebacterium glutamicum base editing method. Metab Eng, 2018, 47: 200-210
16. Zheng X, Zheng P*, Sun J*, Zhang K, Ma Y. Heterologous and endogenous U6 snRNA promoters enabled CRISPR/Cas9 mediated genome editing in Aspergillus niger. Fung. Biol. Biotech, 2018, 5(1):2.
17. Wang Y, Cao G, Xu D, Fan L, Wu X, Ni X, Zhao S, Wang Y, Cao G, Xu D, Fan L, Wu X, Ni X, Zhao S, Zheng P*, Sun J*, Ma Y. A Novel L-Glutamate Exporter of Corynebacterium glutamicum. Appl Enviro Microb, 2018, 84(6): e02691-17.
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承担科研项目情况: |
作为负责人承担20余项科研项目,包括国家重点研发计划课题、国家自然科学基金面上项目、国家973计划项目课题、天津市科技支撑计划、中科院重点部署项目、中科院STS计划项目、中科院知识创新工程项目、企业横向项目等。
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获奖及荣誉: |
2019 中国粮油学会科学技术特等奖(第二位)
2015 天津市劳动模范
2014 中国产学研合作促进奖,中国产学研合作创新成果奖(第一位)
2005 德国GBF基金会优秀博士论文奖
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代表论著: |
1. Qin, J., Zhao, B., Wang, X., Wang, L., Yu, B., Ma, Y., Ma, C., Tang, H., Sun, J., Xu, P. Non-sterilized fermentative production of polymer-grade L-lactic acid by a newly isolated thermophilic strain Bacillus sp. 2-6. PLoS ONE, 2009, 4 (2).
2. Wortman, J.R., Gilsenan, J.M., Joardar, V., …, Sun, J., Taheri-Talesh, N., Takeshita, N., Ussery, D., vanKuyk, P.A., Visser, H., van de Vondervoort, P.J., de Vries, R.P., Walton, J., Xiang, X., Xiong, Y., Zeng, A.P., Brandt, B.W., Cornell, M.J., van den Hondel, C.A., Visser, J., Oliver, S.G., Turner, G. The 2008 update of the Aspergillus nidulans genome annotation: a community effort. Fungal genetics and biology, 2009, 46 Suppl 1, pp. S2-13.
3. Flipphi, M., Sun, J., Robellet, X., Karaffa, L., Fekete, E., Zeng, A.P., Kubiecek, C.P. Biodiversity and evolution of primary carbon metabolism in Aspergillus nidulans and other Aspergillus spp. Fungal genetics and biology, 2009, 46 Suppl 1, pp. S19-S44.
4. Seidl V, Song L, Lindquist E, Gruber S, Koptchinskiy A, Zeilinger S, Schmoll M, Martínez P, Sun J, Grigoriev I, Herrera-Estrella A, Baker SE, Kubicek CP. Transcriptomic response of the mycoparasitic fungus Trichoderma atroviride to the presence of a fungal prey. BMC Genomics. 2009, 10:567.
5. Bizukojc M, Dietz D, Sun J, Zeng AP. Metabolic modelling of syntrophic-like growth of a 1,3-propanediol producer, Clostridium butyricum, and a methanogenic archeon, Methanosarcina mazei, under anaerobic conditions. Bioprocess Biosyst Eng. 2009 Aug 13. [Epub ahead of print]
6. Sun J, Lu X, Rinas U and Zeng AP. Metabolic pecularities of Aspergillus niger disclosed by comparative metabolic genomics. Genome Biology. 8:R182
7. Pel HJ, de Winde JH, Archer DB, Dyer PS, Hofmann G, Schaap PJ, Turner G, de Vries RP, Albang R, Albermann K, Andersen MR, Bendtsen JD, Benen JA, van den BM, Breestraat S, Caddick MX, Contreras R, Cornell M, Coutinho PM, Danchin EG, Debets AJ, Dekker P, van Dijck PW, van Dijk A, Dijkhuizen L, Driessen AJ, D'Enfert C, Geysens S, Goosen C, Groot GS, de Groot PW, Guillemette T, Henrissat B, Herweijer M, van den Hombergh JP, van den Hondel CA, van der Heijden RT, van der Kaaij RM, Klis FM, Kools HJ, Kubicek CP, van Kuyk PA, Lauber J, Lu X, van der Maarel MJ, Meulenberg R, Menke H, Mortimer MA, Nielsen J, Oliver SG, Olsthoorn M, Pal K, van Peij NN, Ram AF, Rinas U, Roubos JA, Sagt CM, Schmoll M, Sun J, Ussery D, Varga J, Vervecken W, van de Vondervoort PJ, Wedler H, Wosten HA, Zeng AP, van Ooyen AJ, Visser J, Stam H (2007). Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88. Nat Biotechnol 25:221-231.
8. Sun J, Wang W, Hundertmark C, Zeng AP, Jahn D, Deckwer WD. (2006) A protein database constructed from low-coverage genomic sequence of Bacillus megaterium and its use for accelerated proteomic analysis. J. Biotechnol. 124:486-495
9. Wang W, Sun J, Hollmann R, Zeng AP, Deckwer WD. (2006) Proteomic characterization of transient expression and secretion of a stress-related metalloprotease in high cell density culture of Bacillus megaterium. J. Biotechnol. 126:313-324.
10.Zheng P, Sun J, van den Heuvel J, Zeng AP. (2006) Discovery and investigation of a new, second triose phosphate isomerase in Klebsiella pneumoniae. J. Biotechnol. 125:462–473
11.Zheng P, Menzel K, Sun J, van den Heuvel J, Zeng AP. (2006) Overexpression of genes of the dha regulon and its effects on cell growth, glycerol fermentation to 1,3-propanediol and plasmid stability in Klebsiella pneumoniae.Process Biochemistry. 41:2160–2169
12.Sun J, Gunzer F, Westendorf AM, Buer J, Scharfe M, Jarek M, G??ling F, Bl?cker H, Zeng AP. (2005) Genomic peculiarity of coding sequences and metabolic potential of the probiotic Escherichia coli strain Nissle 1917 inferred from raw genome sequence.
J. Biotechnol. 117:147–161
13.Sun J, Zeng AP. (2004). IdentiCS - identification of coding sequence and in silico reconstruction of the metabolic network directly from unannotated low-coverage bacterial genome sequence. BMC Bioinformatics. 5:112
14.Sun J, Daniel R, Wagner-D?bler I, and Zeng AP. (2004) Is autoinducer-2 a universal signal for interspecies communication: a comparative genomic and phylogenetic analysis of the synthesis and signal transduction pathways. BMC Evolutionary Biology 4:36
15.Sun J, van den Heuvel J, Soucaille P, Qu Y, Zeng AP. (2003) Comparative genomic analysis of dha regulon and related genes for anaerobic glycerol metabolism in bacteria. Biotechnol Prog. 19:263-272
16.Wang W, Sun J, Hartlep M, Deckwer WD, Zeng AP. (2003) Combined use of proteomic analysis and enzyme activity assays for metabolic pathway analysis of glycerol fermentation by Klebsiella pneumoniae. Biotechnol Bioeng. 83:525-536
17.Wang W*, Sun J*, Nimtz M, Deckwer WD, Zeng AP. (2003) Protein identification from two-dimensional gel electrophoresis analysis of Klebsiella pneumoniae by combined use of mass spectrometry data and raw genome sequences. Proteome Sci. 1:6 *: equal contributor
18.Zeng, A.-P., Sun, J. Continuous Culture, a book chapter in Manual of Industrial Microbiology and Biotechnology, 3rd Ed., edited by R. H. Baltz et al. ASM press, 2010.
19.Sun J and Zeng AP (2008) Systems Biotechnology. In: 2008 Report on the Development of Industrial Biotechnology. Ed. The Bureau of Life Science & Biotechnology, Chinese Academy of Sciences, Science Press, Beijing. (in Chinese)
20.Ma H, Rosa da Silva M,Sun J, Kumar B, Zeng AP (2007) Reconstruction and Structural Analysis of Metabolic and Regulatory Networks. In: "Introduction to Systems Biology". Eds. Sangdun Choi, Humana Press.
21.da Silva MR, Ma H,Sun J, He F, Zeng AP (2006) Metabolic network. In B. H. Junker, F. Schreiber (eds.)Biological Network Analysis, Wiley: 233-253.
22.Zeng, AP;Sun, J; Wang, W; Ma, H; Deckwer, WD (2006) Chapter 2. Application of Genomic and Proteomic Data for Bioprocess Analysis and Optimization. In: Yang, S. T. (ed.)Bioprocessing for value-added products from renewable resources. Elservier Inc,USA, 25-48.
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