副研究员----中国科学院天津工业生物技术研究所

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副研究员

姓名	曲戈	性别	男
专家类别	N/A	职称	副研究员
学历	博士研究生	电话	022-84866457
传真	N/A	电子邮件	qug@tib.cas.cn
地址	天津市空港经济区西七道32号	邮编	300308

简历

2011-2016年，波兰波兹南密茨凯维奇大学，生物信息学，博士

2016-2020年，中国科学院天津工业生物技术研究所，助理研究员

2020年至今，中国科学院天津工业生物技术研究所，副研究员

研究方向：

主要研究领域为酶分子理性设计与生物合成。围绕合成生物学、绿色生物制造等国家战略布局方向，聚焦酶蛋白构效关系解析和高性能酶元件创制等关键科技问题，具体开展：基于生物信息学技术的新酶挖掘与序列重构；利用分子动力学、量子化学等计算技术解析关键酶催化机制；开发酶理性设计改造新方法，提升其原有催化性能或赋予其新功能，并应用于医药中间体、天然产物等功能分子的生物合成等方面工作。

承担科研项目情况：

主持国家自然科学基金2项、国家重点研发计划子课题2项、中国科学院战略性先导C专项课题1项、天津市自然科学基金1项等。

获奖及荣誉：

2021 入选中国科学院青年创新促进会会员 (No. 2021175)

2011 European Union International Ph.D. program “from Genotype to Phenotype” (Consortium Grant MPD 2010/3)

代表论著：

近年来在领域主流期刊Nat. Synth.、Nat. Commun.、Angew. Chem. Int. Ed.、Sci. Bull.、ACS Catal.、Metab. Eng.等发表科技论文60余篇，主编英文专著1部，参编中英书籍4部（章节），申请/授权中国发明专利30余项。

论文（^#共同一作；*通讯作者）

2024

67. Zhang P^#,Yuan B^#,Li J,Li C,Guo J,Zhang B,Qu G,Su H,Turner NJ,Sun Z*. Biocatalytic Desymmetrization for the Atroposelective Synthesisof Axially Chiral Biaryls Using an Engineered Imine Reductase. Angew. Chem. Int. Ed.,2024,e202416569.

66. Li J^#,Yuan B^#,Li C,Zhao Z,Guo J,Zhang P,Qu G,Sun Z*. Stereoselective Synthesis of Oxetanes Catalyzed by an Engineered Halohydrin Dehalogenase. Angew. Chem. Int. Ed.,2024,e202411326.

65. Qin Z,Yuan B,Qu G*,Sun Z*. Rational enzyme design by reducing the number of hotspots and library size. Chem. Commun.,2024,doi: 10.1039/D4CC01394H.

64. Mei Z,Li C,Han X,Tian Y,Li S,Liu W,Qu G,Reetz MT,Sun Z*,Ma J*,Zhang F*. Enzymatic Stereodivergent Access to Fluorinated β-Lactam Pharmacophores via Triple-Parameter Engineered Ketoreductases. ACS Catal.,2024,14,6358-6368.

63. Gong S,Li C,Chu Q,Gao W,Su P,Cui G,Tang J,Qu G,Sun Z*,Guo J*,Huang L*. Rationally Engineered Novel GlycosyltransferaseUGT74DD1 from Siraitia grosvenorii Catalyzes the Generation of the Sweetener Mogroside III. J. Agric. Food Chem.,2024,72,32,18214-18224.

62. Xie P,Lan J,Zhou J,Hu Z,Cui J,Qu G,Yuan B*,Sun Z*. Co-immobilization of amine dehydrogenase and glucose dehydrogenase for the biosynthesis of (S)-2-aminobutan-1-ol in continuous flow. Bioresour. Bioprocess.,2024, 11(1):70.

61. 张晓辉，覃宗敏，李聪聪，路福平，曲戈*，孙周通*．设计改造羧酸还原酶合成医药中间体(S)-2-氨基丁醇．生物学杂志 (J. Biol.)，2024，41(1):6-13.

60. Li X,Li C,Qu G,Yuan B,Sun Z*. Engineering of a Baeyer-Villiger monooxygenase to improve substrate scope,stereoselectivity and regioselectivity. ChemBioChem,2024,e202400328.

59. Ren Y^#,Qin Z^#,Li C,Yuan B,Yang Y,Qu G*,Sun Z*. Engineering the activity and thermostability of a carboxylic acid reductase in the conversion of vanillic acid to vanillin. J. Biotechnol.,2024,386,19-27.

58. Wang Q,Li C,Yuan B,Yu A,Qu G*,Sun Z*. Engineering the activity of a newly identified arylalkylamine N-acetyltransferase in the acetylation of 5-hydroxytryptamine. ChemBioChem,2024,e202400069.

57. Paul C,Hanefeld U,Hollmann F*,Qu G,Yuan B,Sun Z. Enzyme engineering for biocatalysis. Mol. Catal.,2024,555,113874.

56. Ye M,Li C,Xiao D,Qu G,Yuan B*,Sun Z*. Atroposelective Synthesis of Aldehydes via Alcohol Dehydrogenase-Catalyzed Stereodivergent Desymmetrization. JACS Au,2024,4,411-418.

55. Chen L,Qu G,Cai Z*,Yuan B*,Sun Z. Synthesis of a Sacubitril Precursor by Construction of One-pot Chemoenzymatic Cascades. Catal. Sci. Technol.,2024,DOI: 10.1039/D4CY00145A.

54. Yuan B,Yang D,Qu G,Turner NJ*,Sun Z*. Biocatalytic reductive aminations with NAD (P) H-dependent enzymes: enzyme discovery,engineering and synthetic applications. Chem. Soc. Rev.,2024,53,227-262.

53. Reetz MT*,Qu G*,Sun Z*. Engineered enzymes for the synthesis of pharmaceuticals and other high-value products. Nat. Synth.,2024,3,19-32.

2023

52. Li X,Wang J,Su W,Li C,Qu G,Yuan B*,Sun Z*. Characterization and engineering of cephalosporin C acylases to produce 7-Aminocephalosporanic acid. Mol. Catal.,2023,550,113595.

51. Chen Q,Qu G,Li X,Feng M,Yang F,Li Y,Li J,Tong F,Song S,Wang Y*,Sun Z*,Luo G. Active and stable alcohol dehydrogenase-assembled hydrogels via synergistic bridging of triazoles and metal ions. Nat. Commun.,2023,14,2117.

50. Reetz MT*,Sun Z*,Qu G*. Book review of “Enzyme engineering: Selective catalysts for applications in biotechnology,organic chemistry,and life science”. Green Synth. Catal.,2023,4,77.

49. 曲戈*,孙周通*.催化混杂性驱动的酶功能重塑. 生物技术通报(Biotechnol. Bull.),2023,39,1-9.

2022

48. Chen Q,An Y,Feng M,Li J,Li Y,Tong F,Qu G,Sun Z,Wang Y*,Luo G. An enzyme-assembled gel monolithic microreactor for continuous flow asymmetric synthesis of aryl alcohols. Green Chem.,2022,24,9508-9518.

47. 曲戈,袁波,孙周通*．工业蛋白质理性设计与应用.生物工程学报(Chin. J. Biotech.),2022,38,4068-4080.

46. Li J-K^#,Qu G^#,Li X^#,Tian Y,Cui C,Zhang F-G,Zhang W,Ma J-A*,Reetz MT*,Sun Z*. Rational enzyme design for enabling biocatalytic Baldwin cyclization and asymmetric synthesis of chiral heterocycles. Nat. Commun.,2022,13,7813.

45. Jiang Y,Li X,Liu B,Tong F,Qu G*,Sun Z*. Engineering the hydrogen transfer pathway of an alcohol dehydrogenase to increase activity by rational enzyme design. Mol. Catal.,2022,530,112603.

44. Ming H,Yuan B,Qu G*,Sun Z*. Engineering the Activity of Amine Dehydrogenase in the Asymmetric Reductive Amination of Hydroxyl Ketones. Catal. Sci. Technol.,2022,12,5952-5960.

43. Sang X,Tong F,Zeng Z*,Wu M,Yuan B,Sun Z,Sheng X,Qu G,Alcalde M,Hollmann F*,Zhang W*. A Biocatalytic Platform for the Synthesis of Enantiopure Propargylic Alcohols and Amines. Org. Lett.,2022,24,4252-4257.

42. Qin Z,Zhang X,Sang X,Zhang W,Qu G*,Sun Z*. Carboxylic acid reductases enable intramolecular lactamization reactions. Green Synth. Catal.,2022,3,294-297.

41. Wang J^#,Qu G^#,Xie L,Gao C,Jiang Y,Zhang Y-H,Sun Z*,You C*. Engineering of a Thermophilic Dihydroxy acid Dehydratase toward Glycerate Dehydration for in vitro Biosystems. Appl. Microbiol. Biotechnol.,2022,106,3625-3637.

40. Song S^#,Jiang Y^#,Chen R,Su W,Liang W,Yang D,Li J,Zhang W,Gao S,Yuan B*,Qu G*,Sun Z*. Whole-cell Biotransformation of Penicillin G by a Three-enzyme Co-expression System with Engineered Deacetoxycephalosporin C Synthase. ChemBioChem,2022,e202200179.

39. Zhang J,Liao D,Chen R,Zhu F,Ma Y,Gao L,Qu G,Cui C,Sun Z*,Lei X*,Gao S*. Tuning an Imine Reductase for the Asymmetric Synthesis of Azacycloalkylamines by Concise Structure-Guided Engineering. Angew. Chem. Int. Ed.,2022. e202201908.

38. Yang D,Su W,Jiang Y,Gao S,Li X,Qu G*,Sun Z*. Biosynthesis of β-lactam nuclei in yeast. Metab. Eng.,2022,72,56-65.

37. Jiang Y^#,Qu G^#,Sheng X,Tong F,Sun Z*. Unraveling the mechanism of enantio-controlling switches of an alcohol dehydrogenase toward sterically small ketone. Catal. Sci. Technol.,2022,12,1777-1787.

36. 毕悦欣,蒋迎迎,覃宗敏,曲戈*,孙周通*. 醇脱氢酶金属离子绑定位点的可替换性. 生物工程学报(Chin. J. Biotech.),2022,38(4):1518-1526.

35. Bi H^#,Qu G^#,Wang S,Zhuang Y,Sun Z*,Liu T*,Ma Y. Biosynthesis of a rosavin natural product in Escherichia coli by glycosyltransferase rational design and artificial pathway construction. Metab. Eng.,2022,69,15-25.

34. Tong F^#,Qin Z^#,Wang H^#,Jiang Y,Li J,Ming H,Qu G,Xiao Y*,Sun Z*. Biosynthesis of Chiral Amino Alcohols via an Engineered Amine Dehydrogenase in E. coli. Front. Bioeng. Biotechnol.,2022,9:778584.

33. Qu G,Bi Y,Liu B,Li J,Han X,Liu W,Jiang Y,Qin Z,Sun Z*. Unlocking the stereoselectivity and substrate acceptance of enzymes: Proline induced loop engineering test. Angew. Chem. Int. Ed.,2022,61,e202110793.

32. Dong C^#,Qu G^#,Guo J,Wei F,Gao S,Sun Z,Jin L,Sun X,Rochaix J-D,Miao Y*,Wang R*. Rational design of geranylgeranyl diphosphate synthase enhances carotenoid production and improves photosynthetic efficiency in Nicotiana tabacum. Sci. Bull.,2022,67,315-327.

2021

31. Wu R,Yu Y-Y,Wang Y,Wang Y-Z,Song H,Ma C,Qu G,You C,Sun Z,Zhang W,Li A,Li CM*,Yong Y-C*,Zhu Z*. Wastewater-powered high-value chemical synthesis in a hybrid bioelectrochemical system,iScience,2021,24,103401.

30. 张武元,袁波,曲戈,孙周通. 光促酶催化反应设计及生物合成应用. 生物学杂志 (J. Biol.),2021,1-12. 特约综述

29. 张发光,曲戈,孙周通*,马军安*. 从化学合成到生物合成—天然产物全合成新趋势. 合成生物学 (Synbio. J.),2021,2,674-696. 特约评述

28. Chen Q,Guo M,Bi Y,Qu G,Sun Z,Wang Y*,Luo G. Whole-cell biocatalytic synthesis of S-(4-chlorophenyl)-(pyridin-2-yl) methanol in a liquid–liquid biphasic microreaction system. Bioresource Technol.,2021,330: 125022.

27. Li J^#,Qu G^#,Shang N,Chen P,Men Y,Liu W,Mei Z,Sun Y*,Sun Z*. Near-perfect control of the regioselective glucosylation enabled by rational design of glycosyltransferases. Green Synth. Catal.,2021,2,45-53.

2020

26. Wang H^#,Qu G^#,Li JK^#,Ma JA,Guo J,Miao Y,Sun Z*. Data mining of amine dehydrogenases for the synthesis of enantiopure amino alcohols. Catal. Sci. Technol.,2020,10,5945-5952.

25. 蒋迎迎，曲戈，孙周通*. 机器学习助力酶定向进化. 生物学杂志 (J. Biol.),2020,37(4),1-11. 特约综述

24. Mei Z^#,Zhang K^#,Qu G,Li JK,Liu B,Ma JA,Tu R*,Sun Z*. A High-Throughput Fluorescence Assay for Ketone Detection and its Applications in Enzyme Mining and Protein Engineering. ACS Omega,2020,5,13588-13594.

23. Qu G^#,Li A^#,Acevedo-Rocha CG^#,Sun Z*,Reetz MT*. The Crucial Role of Methodology Development in Directed Evolution of Selective Enzymes. Angew. Chem. Int. Ed.,2020,59,13204-13231.

22. Chen J^#,Fan F^#,Qu G^#,Tang J,Xi Y,Bi C,Sun Z*,Zhang X*. Identification of Absidia orchidis steroid 11β-hydroxylation system and its application in engineering Saccharomyces cerevisiae for one-step biotransformation to produce hydrocortisone. Metab. Eng.,2020,57,31-42.

2019

21. 张锟^#，曲戈^#，刘卫东，孙周通. 工业酶结构与功能的构效关系.生物工程学报(Chin. J. Biotech.),2019,35(10):1806-1818.

20. Qu G^#,Liu B^#,Zhang K,Jiang Y,Zhai C,Guo J,Wang R,Miao Y,Sun Z*. Computer-assisted engineering of the catalytic activity of a carboxylic acid reductase. J. Biotechnol.,2019,306,97-104.

19. 曲戈^#，朱彤^#，蒋迎迎，吴边，孙周通*. 蛋白质工程：从定向进化到计算设计. 生物工程学报(Chin. J. Biotech.),2019,35(10),1843-1856. （获得“生物工程学报年度优秀论文奖”）

18 Li A^#,Qu G^#,Sun Z*,Reetz MT*. Statistical Analysis of the Benefits of Focused Saturation Mutagenesis in Directed Evolution Based on Reduced Amino Acid Alphabets. ACS Catal.,2019,9,7769-7778.

17. Liu B^#,Qu G^#,Li J,Fan W,Ma JA,Xu Y,Nie Y*,Sun Z*. Conformational Dynamics-Guided Loop Engineering of an Alcohol Dehydrogenase: Capture,Turnover and Enantioselective Transformation of Difficult-to-Reduce Ketones. Adv. Synth. Catal.,2019,361,3182-3190.

16. Qu G^#,Liu B^#,Jiang Y,Nie Y,Yu H,Sun Z*. Laboratory evolution of an alcohol dehydrogenase towards enantioselective reduction of difficult-to-reduce ketones. Bioresour. Bioprocess. 2019,6(1):18.

15. Sun Z*,Liu Q,Qu G,Feng Y*,Reetz MT*. The Utility of B-Factors in Protein Science: Interpreting Rigidity,Flexibility and Internal Motion and Engineering Thermostability. Chem. Rev.,2019,119,1626-1665.

14. Qu G^#,Fu M^#,Zhao L,Liu B,Liu P,Fan W,Ma J,Sun Z*. Computational Insights into the Catalytic Mechanism of Bacterial Carboxylic Acid Reductase. J. Chem. Inf. Model.,2019,59,832-841.

13. Dai Z^#,Liu Y^#,Sun Z^#,Wang D,Qu G,Ma X,Fan F,Zhang L,Li S,Zhang X*. Identification of a novel cytochrome P450 enzyme that catalyzes the C-2α hydroxylation of pentacyclic triterpenoids and its application in yeast cell factories. Metab. Eng.,2019,51,70-78.

12. 曲戈，张锟，蒋迎迎，孙周通*. 2018诺贝尔化学奖：酶定向进化与噬菌体展示技术. 生物学杂志(J. Biol.),2019,36(1):1-6. 特约综述.

2018

11. Qu G,Guo J,Yang D,Sun Z*. Biocatalysis of carboxylic acid reductases: Phylogenesis,Catalytic Mechanism and Potential Applications. Green Chem.,2018,20(4),777-792.

10. Qu G,Lonsdale R,Yao P,Li G,Liu B,Reetz MT*,Sun Z*. Methodology Development in Directed Evolution: Exploring Options When Applying Triple Code Saturation Mutagenesis. ChemBioChem,2018,19,239-246.

9. Qu G,Zhao J,Zheng P,Sun J,Sun Z*. Recent advances in directed evolution. Chin. J. Biotech.,2018,34(1):1-11.

曲戈，赵晶，郑平，孙际宾，孙周通*。定向进化技术的最新进展。生物工程学报,2018,34(1):1-11. 特邀综述（双语）（获得“生物工程学报年度优秀论文奖”）

8. Yang J^#,Zhu Y^#,Qu G,Zeng Y,Tian C,Dong C,Men Y,Dai L,Sun Z*,Sun Y*,Ma Y. Biosynthesis of dendroketose from different carbon sources using in vitro and in vivo metabolic engineering strategies. Biotechnol. Biofuels,2018,11,290.

Before 2018

7. Qu G,Kruszka K,Plewka P,Yang S-Y,Chiou T-J,Jarmolowski A,Szweykowska-Kulinska Z,Echeverria M*,Karlowski W*. Promoter-based Identification of Novel Non-coding RNAs Reveals the Presence of Dicistronic snoRNA-miRNA Genes in Arabidopsis thaliana. BMC Genomics,2015,16,1009.

6. Wang B,Lu L,Lv H,Jiang H,Qu G,Tian C*,Ma Y. The transcriptome landscape of Prochlorococcus MED4 and the factors for stabilizing the core genome. BMC Microbiol.,2014,14:11.

5. Lv H,Qu G,Qi X,Lu L,Tian C*,Ma Y. Transcriptome analysis of Chlamydomonas reinhardtii during the process of lipid accumulation. Genomics. 2013,101:229-237.

4. Wang M,Jiang Y-Y,Kim KM,Qu G,Ji H-F,Mittenthal JE,Zhang H-Y*,Caetano-Anolles G*. A Universal Molecular Clock of Protein Folds and its Power in Tracing the Early History of Aerobic Metabolism and Planet Oxygenation. Mol. Biol. Evol.,2011,28,567-582.

3. Qu G^#,Wang W^#,Chen L-L,Qian S-S and Zhang H-Y*. tRNA-dependent Cysteine Biosynthetic Pathway Represents a Strategy to Increase Cysteine Contents by Preventing it from Thermal Degradation: Thermal Adaptation of Methanogenic Archaea Ancestor. J. Biomol. Struct. Dyn.,2009,27,111-114.

2. 陈蕾,曲戈. 氧化还原酶辅因子的使用偏好与化学基础,山东农业大学学报(自然科学版),2009,3,393-397.

1. Ma B-G,Che L,Ji H-F,Chen Z-H,Yang F-R,Wang L,Qu G,Jiang Y-Y,Ji C,Zhang H-Y*. Characters of very ancient proteins. Biochem. Biophys. Res. Commun.,2008,366,607-611.

专著/章节：

5. Sun Z,Reetz MT,Qu G. Enzyme Engineering: Selective Catalysts for Applications in Biotechnology,Organic Chemistry,and Life Science. Wiley-VCH,Weinheim,2023. (英文专著)

4. 曲戈,孙周通. 第6章酶的分子改造与修饰. 马延和主编，《高级酶工程》，pp: 139-162,科学出版社,北京,2022. (书籍章节)

3. 曲戈,孙周通. 第3章酶的结构与功能. 马延和主编，《高级酶工程》，pp: 55-83,科学出版社,北京,2022. (书籍章节)

2. Qu G,Sun Z*. In Silico Prediction Methods for Site-Saturation Mutagenesis. In: Magnani F.,Marabelli C.,Paradisi F. (eds),Enzyme Engineering. Methods in Molecular Biology,vol 2397,pp: 49-69,Humana,New York,2022. (书籍章节)

1. Qu G,Sun Z*,Reetz MT*. Iterative Saturation Mutagenesis for Semi-rational Enzyme Design. In: Zhao H. (ed.),Protein Engineering: Tools and Applications,pp: 105-132,Wiley-VCH,Weinheim,2021. (书籍章节)