研究员/正高级工程师
杨建刚

杨建刚


  • 职  称:研究员
  • 学  历:博士研究生
  • 导师类别:博士生导师
  • 通讯地址:天津空港经济区西七道32号
  • 电  话:022-84861961
  • 邮政编码:300308
  • 电子邮件:yang_jg1@tib.cas.cn
  • 部  门:功能糖与天然活性物质研究组

简历

2005.09-2009.07  天津医科大学生物医学工程学院,学士

2009.09-2012.07  天津大学化工学院,硕士

2012.09-2015.07  中国科学院天津工业生物技术研究所,博士

2015.7-2018.11   中国科学院天津工业生物技术研究所,  助理研究员

2018.12-2024.9   中国科学院天津工业生物技术研究所,  副研究员

2024.9-今        中国科学院天津工业生物技术研究所,  研究员

研究方向

研究方向是糖合成生物学,创新非糖质资源依赖人工CO2-糖合成方法,开发灵活性、高效性及多功能性的人工CO2-糖合成平台;模拟自然寡糖和聚糖的合成途径,开发适应工业条件下的糖酶元件,构建体外生物转化系统以及微生物合成细胞工厂,创制具有特殊功能和应用场景的稀少糖、寡糖、多糖等食品配料产品,应用于未来食品等领域。

获奖及荣誉

天津市青年科技人才

中国科学院青年创新促进会会员

天津市自然科学特等奖等奖励2

代表成果

1)  Wang Y.; Chen P.; Li W.; Wang Y.; Dong Q.; Song W.; Liu Y.; Cai T.; Sun Y.; Yang J*.; Ma Y. Cell-free synthesis of high-order carbohydrates from low-carbon molecules. Science Bulletin. 2025. 70, 22662276

2)Wang Y, Li W, Wang D, Zeng Y, Li M, Sun Y,Yang J*. Screening and characterization of thermostable xylose isomerase fromRhodothermus marinusfor erythrose production from one-carbon source.Enzyme Microb Tech.2025, 186, 110607    

3)Liu Y.; Dong Q.; Song W.; Pei W.; Zeng Y.; Wang M.; Sun Y.; Ma Y*.;Yang J*.Microbial synthesis of sedoheptulose from glucose by metabolically engineeredCorynebacterium glutamicum.Microbial Cell Factories. 2024, 23:251

4)Li, J., Li, R., Shang, N., Men, Y., Cai, Y., Zeng, Y., Liu, W., Yang, J*., Sun, Y*. Enzymatic synthesis of novel terpenoid glycoside derivatives decorated with N-acetylglucosamine catalyzed by UGT74AC1. J. Agric. Food Chem. 2024, 72, 25, 14255–14263

5)Wang Y.; Chen E.; Wang Y.; Sun X.; Dong Q.; Chen P.; Zhang C.; Yang J*.; Sun Y. Biosynthesis of mannose from glucose via constructing phosphorylation-dephosphorylation reactions in Escherichia coli. Enzyme Microb Tech. 2024, 177, 110427

6)Liu J.; Li J.; Chen P.; Zeng Y.; Yang J*.; Sun Y*. Efficient production of isomaltulose using engineered Yarrowia lipolytica strain facilitated by non-yeast signal peptide-mediated cell surface display. J. Sci. Food Agric, 2024, 104: 5999 6007

7)Yang, J.; Song, W.; Cai, T.; Wang, Y.; Zhang, X.; Wang, W.; Chen, P.; Zeng, Y.; Li, C.; Sun, Y*.; Ma, Y*. De novo artificial synthesis of hexoses from carbon dioxide. Science Bulletin, 2023.68(20), 2370-2381

8)Sun, X#; Zhang, T#; Liu, Y; Chen, P; Qin, H;Yang, J*; Sun,Y. Self-assembled multienzyme complex facilitates synthesis of glucosylglycerol from maltodextrin and glycerol.J. Sci. Food Agric, 2024, 104(1):266-272

9)Zhang, T#.; Liu, P#.; Wei, H.; Sun, X.; Zeng, Y.; Zhang, X.; Cai, Y.; Cui, M.; Ma, H.;* Liu, W.;* Sun, Y.;*Yang, J*. Protein engineering of glucosylglycerol phosphorylase facilitating efficient and highly regio- and stereoselective glycosylation of polyols in a synthetic system.ACS Catalysis, 2022, 12, 24, 15715–15727.

10)Li,J#.;Mu,S#.; Yang, J*.; Liu, C.; Zhang,Y.;Chen,P.;Zeng,Y.; Zhu,Y.; Sun, Y*. Glycosyltransferase engineering and multi-glycosylation routes development facilitating synthesis of high-intensity sweetener mogrosides. iScience. 2022. 25(10), 105222

11)Tian, C#.;Yang, J#*.;Liu, C#.; Chen, P; Zhang, T.; Men, Y.; Ma, H*.; Sun, Y*.; Ma, Y. Engineering substrate specificity of HAD phosphatases and multienzyme systems development for the thermodynamic-driven manufacturing sugars. Nature Communication. 2022, 13, 3582.

12)Cai, T#.; Sun, H#.; Qiao, J#.; Zhu, L#.; Zhang, F.; Zhang, J.; Tang, Z.; Wei, X.; Yang, J.; Yuan, Q.; Wang, W.; Yang, X.; Chu, H.; Wang, Q.; You, C.; Ma, H.; Sun, Y.; Li, Y.; Li, C.; Jiang, H.; Wang, Q.; Ma, Y*.; Cell-free chemoenzymatic starch synthesis from carbon dioxide, Science, 2021, 372(6562): 1523-152 

13)Ren, C#.;Yang, J*.;Zeng, Y.; Zhang, T.; Tian, C.; Men, Y.; Sun, Y*.Novel catalytic property of fructose-6-phosphate aldolase in directly conversion of two 1-hydroxyalkanones to diketones. Enzyme and Microbial Technology, 2021, 147: 109784

14)Wang, W#.; Yang, J#.; Sun, Y*.; Li, Z*.; You, C*. Artificial ATP-free in vitro synthetic enzymatic biosystems facilitate aldolase-mediated C-C bond formation for biomanufacturing. ACS Catalysis, 2020, 10(2):1264-1271

15)Tian, C#.; Yang, J#*.; Li, Y#.; Zhang, T.; Li, J.; Ren, C.; Men, Y.; Chen, P.; You, C*.; Sun, Y*.; Ma, Y. Artificially designed routes for the conversion of starch to value-added mannosyl compounds through couplingin vitroandin vivometabolic engineering strategies. Metabolic Engineering, 2020, 61:215-224

16)Li, J#.; Yang, J#.; Mu, S.; Shang, N.; Liu, C.; Zhu, Y.; Cai, Y.; Liu, P.; Li, J*.; Liu, W*.; Sun, Y*.; Ma, Y. Efficient O-glycosylation of triterpenes enabled by protein engineering of plant glycosytransferase UGT7AC1. ACS Catalysis, 2020, 10(6):3624-3639

17)Zhang, T#.; Yang, J*.; Tian, C.; Ren, C.; Chen, P.; Men, Y.; Sun, Y*. High-yield biosynthesis of glucosylglycerol through coupling phosphorolysis and transglycosylation reactions. Journal of Agricultural and Food Chemistry, 2020, 68:15249-15256

18)Yang, J#.; Zhang, T.; Tian, C.; Zhu, Y.; Zeng, Y.; Men, Y.; Chen, P.; Sun, Y*.; Ma, Y. Multi-enzyme systems and recombinant cells for synthesis of valuable saccharides: Advances and perspectives. Biotechnology Advances, 2019, 37:107406

19)Yang, J#.; Tian, C.; Zhang, T.; Ren, C.; Zhu, Y.; Zeng, Y.; Men, Y.; Sun, Y*.; Ma, Y. Development of food-grade expression system for d-allulose 3-epimerase preparation with tandem isoenzyme genes in Corynebacterium glutamicum and its application in conversion of cane molasses to D-allulose. Biotechnology and Bioengineering, 2019,116:745-756

20)Men, Y#.; Zhu, P.; Zhu, Y.; Zeng, Y.;Yang, J*.; Sun, Y. The development of low-calorie sugar and functional jujube food using biological transformation and fermentation coupling technology. Food Science & Nutrition,2019, 7:1302–1310.

21)Tian, C#.;Yang, J#.; Zeng, Y.; Zhang, T.; Zhou, Y.; Men, Y.; You, C.; Zhu, Y*.; Sun, Y*. Biosynthesis of raffinose and stachyose from sucrose via anin vitromultienzyme system. Applied and Environmental Microbiology, 2019, 85(2): e02306-18

22)Yang, J#.; Zhu, Y#.; Qu, G.; Zeng, Y.; Tian, C.; Dong, C.; Men, Y.; Dai, L.; Sun, Z*.; Sun, Y*.; Ma, Y. Biosynthesis of dendroketose from different carbon sources using in vitro and in vivo metabolic engineering strategies. Biotechnol Biofuel. 2018. 11, 290. (IF=5.49)

23)Yang, J#.; Sun, S#.; Men, Y.; Zeng, Y.; Zhu, Y.; Sun, Y*.; Ma. Y. Transformation of formaldehyde into functional sugars via multi-enzyme stepwise cascade catalysis.Catal. Sci. Technol.2017. 7, 3459-3463. (IF=5.36)

24)Yang, J#.; Zhu,Y#.; Men, Y.; Sun, S.; Zeng, Y.; Zhang, Y.; Sun, Y*.; Ma, Y. Pathway construction in Corynebacterium glutamicum and strain engineering to produce rare sugars from glycerol. J. Agric. Food Chem. 2016, 64 (50): 9497–9505. (IF=3.41)

25)Yang, J#.; Zhu, Y.; Li, J.; Men, Y.; Sun, Y*.; Ma, Y. Biosynthesis of rare ketoses through constructing a recombination pathway in an engineeredCorynebacterium glutamicum. Biotechnol. Bioeng. 2015. 112, 168–180. (IF=3.95)

26)Yang, J#.; Li, J.; Men, Y.; Zhu, Y.; Zhang, Y.; Sun, Y*.; Ma, Y. Biosynthesis of L-sorbose/psicose based on C-C bond formation catalyzed by aldolases in an engineered Corynebacterium glutamicum strain. Appl. Environ. Microbiol. 2015. 81, 4284-4294. (IF=3.63)

27)Li, J#.; Yang, J#.; Men, Y.; Zeng, Y.; Zhu, Y.; Dong, C.; Sun, Y*.; Ma, Y. Biosynthesis of 2-deoxysugars using whole-cell catalyst expressing 2-deoxy-d-ribose 5-phosphate aldolase. App. Microbiol. Biotech. 2015, 99(19): 7963-7972. (IF=3.34)

28)Yang, J#.; Wang, Z#.; Zhu, N.; Wang, B.; Chen, T*.; Zhao, X.; Metabolic engineering of Escherichia coli and in silico comparing of carboxylation pathways for high succinate productivity under aerobic conditions. Microbiol. Res. 2014. 01. 169(5-6)