2012年至今，天津市生物催化技术工程中心主任

2012年至2014年，中国科学院天津工业生物技术研究所副所长

2009年至2021年，工业酶国家工程实验室副主任

2008年至今，研究员，博士生导师，中国科学院天津工业生物技术所

2004年至2009年，Research Professor, Department of Chemistry, Southern Methodist University, Dallas, Texas, USA

2000年至2004年，Staff Scientist and Group Leader, BioCatalytics, Inc., Pasadena, California, USA

1996年至1999年，Research Associate, University of Houston, Houston, Texas, USA

1994年至1996年，Postdoctoral Associate, Southern Methodist University, Dallas, Texas, USA

1993年至1994年，助理研究员，中国科学院上海有机化学研究所

1993,博士，有机化学，中国科学院上海有机化学研究所

1990, 硕士，有机化学，中国科学院上海有机化学研究所

1987，学士，高分子化学，中国科学技术大学

生物催化与有机合成

1. 生物催化反应机理解析及新反应创建

针对传统化学反应难以实现的碳碳、碳氧和碳氮成键反应，特别是复杂分子手性中心的构建难题，通过解析相关酶的晶体结构、酶催化的反应机理和选择性控制机制，对相关酶进行理性/半理性改造，实现这些难以实现的反应，以及复杂分子手性中心的精准构筑。

2. 系统生物催化与精细化学品的绿色合成

采用有机合成逆合成分析原理，对目标复杂分子进行逆向分析，将传统有机合成方法与生物合成方法交叉融合，创建新型的高效绿色合成路线，实现从简单易得的原料如甲醛到一些重要的天然产物、药物和农药分子的绿色合成。

H. Zhang, X. Chen, T. Lv, J. Feng, X. Liu, P. Yao, Q. Wu, D. Zhu, “Engineering a Carbonyl Reductase to Simultaneously Increase Activity toward Bulky Ketone and Isopropanol for Dynamic Kinetic Asymmetric Reduction via Enzymatic Hydrogen Transfer”, ACS Catalysis, 2023, 13, 9960–9968. https://doi.org/10.1021/acscatal.3c01569

2.   Y. Liu, L. Zhu, Y. Cui, A. Roosta, J. Feng, X. Chen, P. Yao, Q. Wu, D. Zhu, “Photoredox/Enzymatic Catalysis Enabling Redox-Neutral Decarboxylative Asymmetric C–C Coupling for Asymmetric Synthesis of Chiral 1,2-Amino Alcohols”, JACS Au, 2023, https://doi.org/10.1021/jacsau.3c00366

3.   T. Lv, J. Feng, X. Chen, Y. Luo, Q. Wu, D. Zhu, Y. Ma, “Creating novel amine dehydrogenases for precise synthesis of single stereoisomer of β-amino ketones with an all-carbon quaternary stereocenter via desymmetric reductive amination”, ACS Catalysis, 2023, 13, 5053-5061. https://doi.org/10.1021/acscatal.3c00226

4.   Y. Li, N. Hu, Z. Xu, Y. Cui, J. Feng, P. Yao, Q. Wu, D. Zhu, Y. Ma “Asymmetric Synthesis of N-Substituted 1,2-Amino Alcohols via One-Pot Sequential Enzymatic Hydroxymethylation and Asymmetric Reductive Amination”, Angewandt Chemie, International Edition, 2022, 61, e202116344 https://doi.org/10.1002/anie.202116344

5.   N. Liu, L. Wu, J. Feng, X. Sheng, J. Li, X. Chen, J. Li, W. Liu, J. Zhou, Q. Wu, D. Zhu, “Crystal Structures and Catalytic Mechanism of L-erythro-3,5-Diaminohexanoate Dehydrogenase and Rational Engineering for Asymmetric Synthesis of β-Amino Acids”, Angewandt Chemie, International Edition, 2021, 60, 10203–10210 https://doi.org/10.1002/anie.202017225.

6.   P. Yao, J. R. Marshall, Z. Xu, J. Lim, S. J. Charnock, D. Zhu, N. J. Turner, “Asymmetric Synthesis of N-Substituted α-Amino Esters from α-Ketoesters via Imine Reductase-Catalyzed Reductive Amination”, Angewandt Chemie, International Edition, 2021, 60, 8717–8721 https://doi.org/10.1002/anie.202016589 .

7.   S. Yu, J. Li, P. Yao, J. Feng, Y. Cui, J. Li, X. Liu, Q. Wu, J. Lin, D. Zhu, “Inverting the enantiopreference of nitrilase-catalyzed desymmetric hydrolysis of prochiral dinitriles by reshaping the binding pocket with a “mirror image” strategy”, Angewandt Chemie, International Edition, 2021, 60, 3679–3684.  https://doi.org/10.1002/anie.202012243 (selected as Hot Paper).

8.   J. Li, J. Feng, X. Chen, J. Gong, Y. Cui, H. Zhang, D. Bu, Q. Wu, D. Zhu, “Structure-Guided Directed Evolution of a Carbonyl Reductase Enables the Stereoselective Synthesis of (2S,3S)-2,2-Disubstituted-3-hydroxycyclopentanones via Desymmetric Reduction”, Organic Letters, 2020, 22, 3444-3448. https://dx.doi.org/10.1021/acs.orglett.0c00892

9.   N. Liu, J. Feng, R. Zhang, X. Chen, X. Li, P. Yao, Q. Wu, Y. Ma, D. Zhu, “Efficient Microbial Synthesis of Key Steroidal Intermediates from Bio-Renewable Phytosterols by Genetically Modified Mycobacterium fortuitum Strains”, Green Chemistry, 2019, 21, 4076–4083. https://doi.org/10.1039/c9gc01267b

10. X. Chen, H. Zhang, M. A. Maria-Solano, W. Liu, J. Li, J. Feng, X. Liu, S. Osuna, R.-T. Guo, Q. Wu, D. Zhu, Y. Ma, “Efficient Reductive Desymmetrization of Bulky Prochiral 1,3-Cyclodiketone Enabled by Structure-Guided Directed Evolution of a Carbonyl Reductase”, Nature Catalysis, 2019, 2, 931-941. https://doi.org/10.1038/s41929-019-0347-y

11. Q. Chen, X. Chen, J. Feng, Q. Wu, D. Zhu, Y. Ma “Improving and inverting Cb-stereoselectivity of threonine aldolase via substrate-binding-guided mutagenesis and a stepwise visual screening”, ACS Catalysis, 2019, 9, 4462-4469. https://doi.org/10.1021/acscatal.9b00859

12. W. Lu, X. Chen, Y. Wang, J. Feng, Q. Wu, D. Zhu, “A Fungal P450 from Thanatephorus cucumeris with Novel Steroid Hydroxylation Capabilities”, Applied and Environmental Microbiology, 2018, 84: e00503-18.  https://doi.org/10.1128/AEM.00503-18 (selected as Spotlight Article by Editors).

13. X. Chen, H. Zhang, J. Feng, Q. Wu, D. Zhu, “Molecular basis for the high activity and enantioselectivity of carbonyl reductase from Sporobolomyces salmonicolor toward α-halo-acetophenones”, ACS Catalysis, 2018, 8, 3525-3531. https://doi.org/10.1021/acscatal.8b00591

14. P. Yao, P. Cong, R. Gong, J. Li, G. Li, J. Ren, J. Feng, J. Lin, P. C. K. Lau, Q. Wu, D. Zhu, “Biocatalytic Route to Chiral 2-Substituted-1, 2, 3, 4-tetrahydroquinolines Using Cyclohexylamine Oxidase Muteins”, ACS Catalysis, 2018, 8, 1648-1652. https://doi.org/10.1021/acscatal.7b03552

15. J. Ren, P. Yao, S. Yu, W. Dong, Q. Chen, J. Feng, Q. Wu, D. Zhu, “An Unprecedented Effective Enzymatic Carboxylation of Phenols”, ACS Catalysis, 2016, 5, 564-567. https://doi.org/10.1021/acscatal.5b02529

16. D. Zhang, X. Chen, R. Zhang, P. Yao, Q. Wu, D. Zhu, “Development of β-Amino Acid Dehydrogenase for the Synthesis of β-Amino Acids via Reductive Amination of β-Keto Acids”, ACS Catalysis, 2015, 5, 2220-2224. https://doi.org/10.1021/cs5017358