王猛
1、教育经历
2001.9-2005.7,浙江大学,化学,学士学位
2001.9-2005.7,浙江大学,生物信息学,第二学位
2005.8-2009.12, 雪城大学(Syracuse University),化学,博士学位
2、工作经历
2010.1-2015.4,伊利诺伊大学厄巴纳 - 香槟分校(University of Illinois at Urbana-Champaign),博士后
2015-至今, 中国科学院天津工业生物技术研究所,研究员
1.Ran Tu, Yue Zhang, Erbing Hua, Likuan Bai, Huamei Huang, Kaiyue Yun, Meng Wang*,(2021)Droplet-based microfluidic platform for high-throughput screening of Streptomyces. Commun Biol, Accepted
2.卢挥张启于思礼王钰康明韩双艳刘叶* 王猛,(2021)谷氨酸棒杆菌中基于CRISPR/Cas9的多位点碱基编辑系统的优化 .生物工程学报
3.Yue Zhang, Kaiyue Yun, Huamei Huang, Ran Tu, Erbing Hua*, Meng Wang*,(2021)Antisense RNA Interference-Enhanced CRISPR/Cas9 Base Editing Method for Improving Base Editing Efficiency in Streptomyces lividans 66. ACS Synth Biol. doi: 10.1021/acssynbio.0c00563
4.Yu Wang*, Haijiao Cheng, Yang Liu, Ye Liu, Xiao Wen, Kun Zhang, Xiaomeng Ni, Ning Gao, Liwen Fan, Zhihui Zhang, Jiao Liu, Jiuzhou Chen, Lixian Wang, Yanmei Guo, Ping Zheng*, Meng Wang*, Jibin Sun and Yanhe Ma,(2021) In-situ generation of large numbers of genetic combinations for metabolic reprogramming via CRISPR-guided base editing. Nat Commun, 12(1):678.
5.Yu Wang*, Ye Liu, Ping Zheng, Jibin Sun and Meng Wang*,(2020) Microbial Base Editing: A Powerful Emerging Technology for Microbial Genome Engineering. Trends Biotechnol, S0167-7799(20)30174-8
6.Sili Yu, Marcus A. Price Yu Wang, Yang Liu, Yanmei Guo, Xiaomeng N, Susan J. Rosser, Changhao Bi* and Meng Wang*, (2020) CRISPR-dCas9 mediated cytosine deaminase base editing in Bacillus subtilis. ACS Synth Biol. 9(7):1781-1789
7.黄华媚白立宽刘叶李俊维王猛* 花尔并* ,(2019) BE3 型胞嘧啶碱基编辑器在谷氨酸棒杆菌中的开发及应用. 生物技术通报 36(3)
8.Xiaoting Lan, Wei Yuan, Meng Wang*, Han Xiao*. (2019). Efficient biosynthesis of antitumor ganoderic acid HLDOA using a dual tunable system for optimizing the expression of CYP5150L8 and a Ganoderma P450 reductase. Biotechnol Bioeng 116(12):3301-3311
9.李俊维刘叶王钰郁彭郑平王猛*, (2019) 谷氨酸棒杆菌碱基编辑的条件优化, 生物工程学报 DOI:10.13345/j.cjb.190192
10.Yu Wang, Ye Liu, Junwei Li,1, Yi Yang, Xiaomeng Ni, Haijiao Cheng, Teng Huang, Yanmei Guo, Hongwu Ma, Ping Zheng*, Meng Wang*, Jibin Sun, Yanhe Ma, (2019). Expanding targeting scope, editing window, and base transition capability of base editing in Corynebacterium glutamicum. Biotechnol Bioeng, 116(11):3016-3029
11. Han Xiao*, Yue Zhang#, Meng Wang*,(2018)Discovery and engineering of cytochrome P450s for terpenoid biosynthesis,Trends Biotechnol. pii: S0167-7799(18)30318-4
12.崔金明张炳照马迎飞傅雄飞王猛刘陈立*,(2018)合成生物学研究的工程化平台,中国科学院院刊11:1249-1257
13. Graham Heberlig, Jesse T. C. Brown, Ryan Simard, Monica Wirz, Wei Zhang, Meng Wang, Leah I Susser, Mark E Horsman, Christopher Noyce Boddy*, (2018) Chemoenzymatic Macrocycle Synthesis Using Resorcylic Acid Lactone Thioesterase Domains. Org. Biomol. Chem. DOI:10.1039/C8OB01512K
14. Yu W, Ye L, Jiao L, Yanmei G, Liwen F, Xiaomeng N, Xiaomei Z , Meng W*, Ping Z*, Jibin S*, Yanhe M,(2018)MACBETH: multiplex automated Corynebacterium glutamicum base editing method. Metabolic Engineering. May 47:200-210
15. Yang L, Ye L, Meng W* (2017). Design, optimization and application of small molecule biosensor in metabolic engineering. Frontiers in Microbiology. Oct 17;8:2012
16. Wang, M., Zhao, H. (2016). Combined and Iterative Use of Computational Design and Directed Evolution for Protein-Ligand Binding Design. Methods in Molecular Biology. 1414:139-53.
17. Wang, M., Yu, C., Zhao, H. (2016). Identification of an important motif that controls the activity and specificity of sugar transporters. Biotechnology and Bioengineering.113(7):1460-7
18. Wang, M., Yu, C., Zhao, H. (2016). Directed evolution of xylose specific transporters to facilitate glucose-xylose co-utilization. Biotechnology and Bioengineering. 113(3):484-91
19. Li, S., Si, T., Wang, M., Zhao, H. (2015). Development of a Synthetic Malonyl-CoA Sensor in Saccharomyces cerevisiae for Intracellular Metabolite Monitoring and Genetic Screening. ACS synthetic biology. DOI:10.1021/acssynbio.5b00069
20. Wang, M., Li, S., Zhao, H. (2015). Design and engineering of intracellular-metabolite-sensing/regulation gene circuits in Saccharomyces cerevisiae. Biotechnology and Bioengineering.DOI: 10.1002/bit.25676
21. Wang, M., Zhao, H. (2014) Characterization and engineering of the adenylation domain of a NRPS-like protein: a potential biocatalyst for aldehyde generation. ACS catalysis, 4(4), 1219-1225.
22. Heberlig, G.W., Wirz, M., Wang, M., and Boddy, C. N. (2014). Resorcylic acid lactone biosynthesis relies on a stereo-tolerant macrocyclizing thioesterase. Organic Letters, 16(22), 5858-5861.
23. Wang, M., Beissner, M.; Zhao, H.(2013)Aryl-aldehyde formation in fungal polyketides: Discovery and characterization of a distinct biosynthetic mechanism. Chemistry & Biology.21(2), 257-263.
24. Luo, Y., Huang, H., Liang, J., Wang, M., Lu, L., Shao, Z., Cobb, R.E., and Zhao, H. (2013)Activation and characterization of a cryptic polycyclic tetramate macrolactam biosynthetic gene cluster. Nature Communications. 4, 2894.
25. Pinto, A., Wang, M., Horsman, M., Boddy, C. N. (2012) 6-Deoxyerythronolide B synthase thioesterase-catalyzed macrocyclization is highly stereoselective. Organic Letters, 14(9), 2278-2281.
26. Wang, M., Si, T. and Zhao, H. (2011) Biocatalyst development by directed evolution. Bioresource Technology, 115,117-125.
27. Wang, M. and Zhao, H. (2011) Microbial synthesis of drugs and fuels via synthetic biology. Chinese Bulletin of Life Sciences, 23, 875-881.
28. Wang, M., Zhou, H., Wirz, M., Tang Y. and Boddy, C.N. (2009) A thioesterase from an iterative fungal polyketide synthase shows macrocyclization and cross coupling activity and may play a role in controlling iterative cycling through product offloading. Biochemistry 48 (27), 5288–6290.
29. Wang, M., Opare, P. and Boddy, C. N. (2009) Polyketide synthase thioesterases catalyze rapid hydrolysis of peptidyl thioesters. Bioorganic and Medicinal Chemistry Letter 19 (5), 1413-1415.
30. Wang, M. and Boddy, C. N. (2008) Examining the role of hydrogen bonding interactions in the substrate specificity for the loading step of polyketide synthase thioesterase domains. Biochemistry 47 (45), 11793–11803.
31. Wu, Q., Wang, M., Chen, Z. C., Lu, D. S., Lin, X. F. (2006) Enzymatic synthesis of metronidazole esters and their monosaccharide ester derivatives. Enzyme Microbiology and Technology 39 (6), 1258-1263.
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