柏文琴
2010-2013年,中国科学院微生物研究所,博士
2014-2015年,中国科学院天津工业生物技术研究所,助理研究员
2015-2019年,明尼苏达大学和圣路易斯华盛顿大学,访问学者,博士后
2020年-至今,中国科学院天津工业生物技术研究所,研究员,博士生导师
主要利用合成生物学和代谢工程技术,围绕重要工业酶、医用蛋白及活性聚合物等生物大分子的微生物合成开展基础及应用研究。
在Nature Communications、Metabolic Engineering、Carbohydrate Polymers、Microbial Cell Factories等期刊发表论文43篇,申请专利23项,授权12项,成果转化2项, 参编教材两本,专著一部。
1. Jiaqi Gao, Zijun Hao, Jie Zhen, Wenqin Bai*. Transcriptomics and Experimental Analysis Reveal the Molecular Basis of pH-Dependent Pullulan Biosynthesis in Aureobasidium pullulans BL06ΔPMAs. In preparation.
2. Zhaoxuan Feng, Shuyu Chen, Jie Zhen, Wenqin Bai*. Shrinkable elastic hydrogel for solvent-induced resolution enhancement in 3D printing. In preparation.
3. Zheng Hu, Marwa Abou-Taleb, Jianyong Xu, Hui Song, Salwa Mowafi, Hongchen Zheng, Wenqin Bai*, Hosam El-Sayed*. A New Hydrogen Peroxide-Tolerant Alkaline Protease as a Benign Element for Simultaneous Bleaching and Felt-Proofing of Wool. Sustainable Chemistry and Pharmacy. major revision.
4. Qingfang Meng, Dexin Wang, Xiaoping Fu, Weitao Geng*, Hongchen Zheng*, Wenqin Bai*. Converting Bacillus subtilis 168 to a synthetic methylotroph by combinatorial metabolic regulation strategies. J. Agric. Food Chem. 2025, 73(8):4755-4763.
5. Jiaqi Gao, Xiaoping Liao, Hongwu Ma, Wenqin Bai*. Transcriptome analysis of Aureobasidium pullulans BL06 and identification of key factors affecting pullulan production. Carbohydrate Polymers. 2025, 349(Pt A):122984.
6. Senqin Hou, Shibin Yang, Wenqin Bai*. Multi-gene precision editing tool using CRISPR-Cas12a/Cpf1 system in Ogataea polymorpha. Microbial Cell Factories. 2025, 24(1):28.
7. Huizhi Liang, Wenkang Dong, Xiaoping Fu, Hongchen Zheng, Ming Li, Wenqin Bai*. High-efficiency secretory expression of polyethylene terephthalate hydrolases in Bacillus amyloliquefaciens 205 and complete degradation of PET by its crude enzyme. Int J Biol Macromol. 2025, 330(Pt 2):148111.
8. Yi Hu, Yu Duan, Yuan Zhang, Longxue Ma, Yida Zhai, Wuxi Chen, Liucheng Long, Wenqin Bai, Yanbing Shen, Limei Chen*, Xiaoqing Ren*, Demao Li*. Reprogramming Schizochytrium cell factory for High-Yield production of astaxanthin via mutagenesis and metabolic engineering. Chemical Engineering Journal. 2025, 515:163556.
9. Aijia Shi, Menghan Chi, Zhen Zhu, Wenqin Bai, Jinling Cai, Limei Chen* & Demao Li*. Screening and fermentation optimization of Methylophilus sp. HN238 for efficient microbial protein production using methanol. Systems Microbiology and Biomanufacturing. 2025, 5:1050–1066.
10. Meijuan Zhang, Jie Zhen, Jia Teng, Xingya Zhao, Xiaoping Fu, Hui Song, Yeni Zhang, Hongchen Zheng*, Wenqin Bai*. N-Terminal Sequences of Signal Peptides Assuming Critical Roles in Expression of Heterologous Proteins in Bacillus subtilis. Microorganisms.2024, 2(7):1275.
11. Dexin Wang, Xiaoping Fu, Jiaqi Gao, Xingya Zhao, Wenqin Bai*. Enhancing Poly-γ-glutamic Acid Production in Bacillus tequilensis BL01 through a Multi enzyme Assembly Strategy and Expression Features of Glutamate Synthesis from Corynebacterium glutamicum. J Agric Food Chem. 2024, 72(15):8674-8683.
12. Wenxin Huang, Sijia Wang, Zhaoxuan Feng, Dasen Zhou, Wenqin Bai*. Tyrosinase-Modified UHMW SELP Polymers as Wet and Underwater Adhesives to Achieve Multi-interface Adhesion. ACS Synth. Biol. 2024, 13, 4, 1191-1204.
13. Zhaoxuan Feng, Sijia Wang, Wenxin Huang, Wenqin Bai*. A potential bilayer skin substitute based on electrospun silk-elastin-like protein nanofiber membrane covered with bacterial cellulose. Colloids and Surfaces B: Biointerfaces. 2024, 234:113677.
14. Xingya Zhao, Xiaoping Fu, Jie Zhen, Jun Yang, Hui Song*, Hongchen Zheng*, Wenqin Bai*. BsuMI regulates DNA transformation in Bacillus subtilis besides the defense system and the constructed strain with BsuMI-absence is applicable as a universal transformation platform for wild-type Bacillus. Microbial Cell Factories. 2024, 23(1):225.
15. Meijuan Zhang, Xiaoping Fu, Rongrong Gu, Bohua Zhao, Xingya Zhao, Hui Song, Hongchen Zheng*, Jianyong Xu*, Wenqin Bai*. A novel starch-active lytic polysaccharide monooxygenase discovered with bioinformatics screening and its application in textile desizing. BMC Biotechnology 2024, 24(1):2.
16. Sijia Wang†, Wenxin Huang†, Zhaoxuan Feng, Xiaoli Tian, Dexin Wang, Lang Rao, Ming Tan, Niran Roongsawang, Hui Song*, Wenxia Jiang*, Wenqin Bai*. Laccase-mediated formation of hydrogels based on silk-elastin-like protein polymers with ultra-high molecular weight. International Journal of Biological Macromolecules. 2023, 231:123239.
17. Shuyu Chen†, Hongchen Zheng†, Jiaqi Gao, Hui Song, Wenqin Bai*. High-level production of pullulan and its biosynthesis regulation in Aureobasidium pullulans BL06. Frontiers in Bioengineering and Biotechnology. 2023, 11:1131875.
18. Xi Sun, Jun Yang, Xiaoping Fu, Xingya Zhao, Jie Zhen, Hui Song, Jianyong Xu, Hongchen Zheng*, Wenqin Bai*. Trehalose Production Using Three Extracellular Enzymes Produced via One-Step Fermentation of an Engineered Bacillus subtilis Strain. Bioengineering-Basel. 2023, 10(8):977.
19. Yifan Yang, Xiaoping Fu, Xingya Zhao, Jianyong Xu, Yihan Liu*, Hongchen Zheng*, Wenqin Bai*, Hui Song. Overexpression of a thermostable α-amylase through genome integration in Bacillus subtilis. Fermentation-Basel. 2023, 9(2):139.
20. Dexin Wang, Xiaoping Fu, Dasen Zhou, Jiaqi Gao, Wenqin Bai*. Engineering of a newly isolated Bacillus tequilensis BL01 for poly-γ- glutamic acid production from citric acid. Microbial Cell Factories. 2022, 21(1):276.
21. Zhaoxuan Feng, Jinsong Li, Dasen Zhou, Hui Song, Jiaqi Lv*, Wenqin Bai*. A novel photocurable pullulan-based bioink for digital light processing 3D printing. International Journal of Bioprinting. 2023, 9(2): 657.
22. Zhaoxuan Feng†, Shuyu Chen†, Abdullah Ahmad, Longjiao Chen, Wenqin Bai*. Ultra-high molecular weight pullulan-based material with high deformability and shape-memory properties. Carbohydrate Polymers. 2022, 295:119836.
23. Wenqin Bai†, Winston E. Anthony†, Christopher Hartline, Shaojie Wang, Bin Wang, Jie Ning, Fong-Fu Hsu, Gautam Dantas*, Fuzhong Zhang*. Engineering Diverse Fatty Acid Compositions of Phospholipids in Escherichia coli. Metabolic Engineering. 2022, 74:11-23.
24. Wenqin Bai, Cameron J. Sargent, Jeong-Mo Choi, Rohit V. Pappu, Fuzhong Zhang*. Covalently-assembled single-chain protein nanostructures with ultra-high stability. Nature Communications. 2019, 10, 3317.
25. Wenqin Bai, Weitao Geng, Shaojie Wang and Fuzhong Zhang*. Biosynthesis, regulation, and engineering of microbially produced branched biofuels. Biotechnology for Biofuels. 2019, 12:84.
26. Christopher H. Bowen, Bin Dai, Cameron J. Sargent, Wenqin Bai, Pranay Ladiwala, Huibao Feng, Wenwen Huang, David L. Kaplan, Jonathan M. Galazka, Fuzhong Zhang*. Recombinant spidroins fully replicate primary mechanical properties of natural spider silk. Biomacromolecules, 2018, 19, 3853-3860.
27. Wenqin Bai, Yi-Shu Tai, Jingyu Wang, Jilong Wang, Pooja Jambunathan, Kevin J. Fox, Kechun Zhang*. Engineering Nonphosphorylative Metabolism to Synthesize Mesaconate from D-Xylose and L-Arabinose in Escherichia coli. Metabolic Engineering, 2016 (38): 285–292.
28. Wenqin Bai*, Yufan Cao, Jun Liu, Qinhong Wang, Zhenhu Jia. Improvement of alkalophilicity of an alkaline xylanase Xyn11A-LC from Bacillus sp. SN5 by random mutation and Glu135 saturation mutagenesis. BMC Biotechnology, 2016(16):77
29. Wenqin Bai, Yanfen Xue, Yueju Zhao, Qinhong Wang, Yanhe Ma*. Structural insight into and mutational analysis of family 11 xylanases: implications for mechanisms of higher pH catalytic adaptation. PLoS One. 2015, Jul 10, 10(7):e0132834.
30. Wenqin Bai, Yanfen Xue, Cheng Zhou, Yanhe Ma*. Cloning, expression and characterization of an alkali-tolerant xylanase from alkalophilic Bacillus sp. SN5. Biotechnol Appl Biochem. 2015, 62(2):208-17.
31. Wenqin Bai, Cheng Zhou, Yanfen Xue, Chunhsiang Huang, Ruiting Guo, Yanhe Ma*. Three-dimensional structure of an alkaline xylanase Xyn11A-LC from alkalophilic Bacillus sp. SN5 and improvement of its thermal performance by introducing arginines substitutions. Biotechnol Lett. 2014, 36(7):1495-501.
32. Wenqin Bai, Yanfen Xue, Cheng Zhou, Yanhe Ma*. Cloning, expression and characterization of a novel salt-tolerant xylanase from Bacillus sp. SN5. Biotechnol Lett. 2012, 34(11):2093-9.
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