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研究员/正高级工程师
张燕飞

张燕飞

简历

2010 – 2015年，加拿大阿尔伯塔大学（University of Alberta）生物化学系，博士 

2015 – 2020年，美国普林斯顿大学（Princeton University），博士后 

2020 – 至今，中国科学院天津工业生物技术研究所，研究员 

研究方向

  • 蛋白质工程与合成进化 (Protein engineering & Synthetic evolution)
  • 合成生物学与代谢工程(Synthetic biology & Metabolic engineering)
  • 合成系统生物学 (Synthetic system biology)  

    • 研究组的使命是探寻生物技术方案，以应对和解决能源、环境、生物制造和人类健康方面的挑战和需求。为了实现这一目标，我们整合了微生物学、分子生物学、遗传学、细胞生物学、生物化学、生物物理学、结构生物学和工程学等多种学科，从合成生物学与代谢工程的角度对微生物群落、细胞、细胞器和蛋白质进行改造。

获奖及荣誉

  • JBCollip生物化学学生奖学金，2014
  • Andrew Stewart 研究生纪念奖学金，2013
  • 阿尔伯塔大学医学与牙科学院/阿尔伯塔省健康服务厅研究生新生奖学金，2011

代表成果

  1. Meizi Liu*,Zuhui Li*,Jianfeng Huang*,Junjun Yan,Guoping Zhao#,Yanfei Zhang# OptoLacI: Optogenetically Engineered Lactose Operon Repressor LacI Responsive to Light instead of IPTG. Nucleic Acids Research 2024 1-14 https://doi.org/10.1093/nar/gkae479

  1. Qian Chen,Yunhong Chen,Zeming Hou,Yuyue Ma,Jianfeng Huang,Zhidan Zhang,Yefu Chen,Xue Yang#,Yanfei Zhang#,Guoping Zhao#. Unlocking the formate utilization of wild-type Yarrowia lipolytica through adaptive laboratory evolution. Biotechnology Journal 20242400290. https://doi.org/10.1002/biot.202400290

  1. Li Zhang*,Ling Tan*,Meizi Liu,Yunhong Chen,Yu Yang#,Yanfei Zhang#,Guoping Zhao. Quantitative measurement of cell-surface displayed proteins based on split-GFP assembly. Microbial Cell Factories 2024 23:108.  https://doi.org/10.1186/s12934-024-02386-1

  1. Jianfeng Huang,Jinhui Liu,Huaming Dong,Jingjing Shi,Xiaoyan You#,and Yanfei Zhang#. Engineering of a substrate affinity reduced S-adenosyl-methionine synthase as a novel biosensor for growth-coupling selection of L-methionine overproducers,Applied Biochemistry and Biotechnology 2024 Vol. 196.  https://doi.org/10.1007/s12010-023-04807-0

  1. Yang,Xue,Zhang,Yanfei#,& Zhao,Guoping#. Artificial carbon assimilation: From unnatural reactions and pathways to synthetic autotrophic systems. Biotechnology Advances 70 (2024) 108294.  https://doi.org/10.1016/j.biotechadv.2023.108294

  1. Ling Tan,Meizi Liu,Lixian Wang,Guoping Zhao,Yanfei Zhang,Flow cytometry-based high-throughput screening of synthetic peptides for palladium adsorption,Journal of Hazardous Materials 2024 Vol. 461 Pages 132656.  https://doi.org/10.1016/j.jhazmat.2023.132656

  1. Yang,X.#,Mao,Z.,Huang,J.,Wang,R.,Dong,H.,Zhang,Y#.,& Ma,H#. Improving pathway prediction accuracy of constraints-based metabolic network models by treating enzymes as microcompartments. Synthetic and systems biotechnology vol. 8,4 597-605. 12 Sep. 2023. https://doi.org/10.1016/j.synbio.2023.09.002
  2. Zuhui Li*,Ran Ge*,Ling Tan,Wuyuan Zhang#,and Yanfei Zhang#, Photodecarboxylase Displayed on Microbial Cell-Surface for Enhanced Biocatalytic Performance. ChemCatChem 2023,e202300494.  https://doi.org/10.1002/cctc.202300494

  1. Dong,Huaming*,Xue Yang*,Jingjing Shi,Chunqiao Xiao#,and Yanfei Zhang#. Exploring the Feasibility of Cell-Free Synthesis as a Platform for Polyhydroxyalkanoate (PHA) Production: Opportunities and Challenges. Polymers 15,(2023) no. 10: 2333.

  1. Ling Tan, Xindi Liu, Yanfei Zhang.Glutaraldehyde fixation promotes palladium and gold nanoparticles formation in yeast and enhances their catalytic activity in 4-nitrophenol reduction.Journal of Hazardous Materials Volume 446, 15 March 2023, 130696.  https://doi.org/10.1016/j.jhazmat.2022.130696

  1. Zhang Y., Cortez, J.D. Hammer S.K., Carrasco-Lopez C., Garcia Echauri S.A.,Wiggins J.B., Wang W., Avalos J.L. Biosensor for branched-chain amino acid metabolism in yeast and applications in isobutanol and isopentanol production. Nature Communications 13, 270 (2022). https://doi.org/10.1038/s41467-021-27852-x

  1. Scott A Wegner, Jhong-Min Chen, Samantha S Ip, Yanfei Zhang, Deepak Dugar, José L Avalos, Engineering acetyl-CoA supply and ERG9 repression to enhance mevalonate production in Saccharomyces cerevisiae, Journal of Industrial Microbiology and Biotechnology, (2021) . https://doi.org/10.1093/jimb/kuab050

  1. Zhang Y., Lane, S., Chen, J.-M., Hammer, S.K., Luttinger, J., Yang, L., Jin, Y.-S., Avalos, J.L. (2019) Xylose utilization stimulates mitochondrial production of isobutanol and 2-methyl-1-butanol in Saccharomyces cerevisiae. Biotechnology for Biofuels. 12(1):223. https://doi.org/10.1186/s13068-019-1560-2

  1. Sarah K.H., Zhang Y., and Avalos J.L. (2020) Mitochondrial compartmentalization confers specificity to the 2-ketoacid recursive pathway: increasing isopentanol production in Saccharomyces cerevisiae.ACS Synth. Biol. 9, 3, 546-555.  

  1. Álvarez M.S., Zhang Y.. (2019) Sketching neoteric solvents for boosting drugs bioavailability. Journal of Controlled Release. 311-312: 225-232
  2. Lane S., Zhang Y., Yun E.J., Ziolkowski L., Zhang G., Jin Y.-S., Avalos J.L. (2019) Xylose assimilation enhances production of isobutanol in engineered Saccharomyces cerevisiae. Biotechnology and Bioengineering. 117:372–381. DOI: 10.1002/bit.27202
  3. Zhao E.M., Zhang Y., Mehl J., Park H., Lalwani M.A., Toettcher J.E., Avalos J.L. (2018) Optogenetic regulation of engineered cellular metabolism for microbial chemical production. Nature, 555: 683-687. https://doi.org/10.1038/nature26141

  1. Zhang Y, Avalos J.L. (2017) Traditional and novel tools to probe the mitochondrial metabolism in health and disease. WIREs Syst Biol Med. doi: 10.1002/wsbm.1373

  1. Zhang Y., Qadri A. and Weiner J.H. (2015) The quinone-binding site of Acidithiobacillus ferrooxidans sulfide:quinone oxidoreductase controls both sulfide oxidation and quinone reduction. Biochemistry and Cell Biology, 10.1139/bcb-2015-0097

  1. Zhang Y., and Weiner J.H. (2014) Characterization of the kinetics and electron paramagnetic resonance spectroscopic properties of Acidithiobacillus ferrooxidans Sulfide:Quinone Oxidoreductase (SQR). Archives of Biochemistry and Biophysics 564:110–119

  1. Zhang Y., and Weiner J.H. (2014) A simple semi-quantitative in vivo method using H2S detection to monitor sulfide metabolizing enzymes. BioTechniques 57:208-210.

  1. Zhang, Y., Yang, Y., Liu, J., and Qiu, G. (2013) Isolation and Characterization of Acidithiobacillus ferrooxidans Strain QXS-1 Capable of unusual Ferrous Iron and Sulfur Utilization. Hydrometallurgy. 136:51-57

  1. Zhang, Y., Peng, A., Yang, Y., Liu, J., and Qiu, G. (2013) Isolation, characterization of Acidiphilium sp. DX1-1 and ore bioleaching by this acidophilic mixotrophic organism. Trans Nonferrous Met Soc. China. 23:1774-1782

  1. Cherney, M.M., Zhang, Y., James, M.J. and Weiner, J.H.(2012) Structure-activity characterization of sulfide:quinone oxidoreductase variants. Journal of Structural Biology, 178: 319-328.

  1. Cherney M.M.*, Zhang Y.*, Solomonson M., Weiner J.H., James M.N. (2010) Crystal structure of sulfide:quinone oxidoreductase from Acidithiobacillus ferrooxidans: insights into sulfidotrophic respiration and detoxification. J Mol Biol 398:292-305

(* Cherney M.M. and Zhang Y. contributed equally to this study.)

  1. Zhang Y., Cherney M.M., Solomonson M., Liu J., James .MN., Weiner J.H. (2009) Preliminary X-ray crystallographic analysis of sulfide:quinone oxidoreductase from Acidithiobacillus ferrooxidans. Acta Crystallogr Sect F Struct Biol Cryst Commun 65:839-842
  2. Zheng C., Zhang Y., Liu, Y.Wu, A.Xia, L.Zeng, J.Liu, J.Qiu, G. (2009) Characterization and reconstitute of a [Fe4S4] adenosine 5'-phosphosulfate reductase from Acidithiobacillus ferrooxidans. Curr Microbiol 58:586-592
  3. Zeng L., Huang J., Zhang Y., Qiu G., Tong J., Chen D., Zhou J., Luo X. (2008) An effective method of DNA extraction for bioleaching bacteria from acid mine drainage. Appl Microbiol Biotechnol 79:881-888
  4. Wu A., Zhang Y., Zheng C., Dai Y., Liu Y., Zeng J. (2008) Purification and enzymatic characteristics of cysteine desulfurase, IscS, in Acidithiobacillus ferrooxidans ATCC 23270. Trans. Nonferrous Met. Soc. China 18:1450-1457
  5. Yang Y., Shi W., Wan M., Zhang Y., Zou L., Huang J., Qiu G. and Liu X. (2008) Diversity of bacterial communities in acid mine drainage from the Shen-bu copper mine, Gansu province, China. Electron. J. Biotechnol.11:108–117
  6. Zeng J.*, Zhang Y.*, Liu Y., Zhang X., Xia L., Liu J. and Qiu G. (2007) Expression, purification and characterization of a cysteine desulfurase, IscS, from Acidithiobacillus ferrooxidans. Biotechnology Letters29(12):1983-90

(*Yanfei Zhang and Jia Zeng contributed equally to this work)

  1. Zhang Y., Yang Y., Liu J. and Qiu G. (2007) Isolation, Characterization and Phylogenetic Analysis of Acidiphilium-like Bacteria from Acid Mine Drainage. Advanced Materials Research Vols. 20-21:473-476
  2. Zhang Y., Zhang X., Wang Y., Yang Y., Liu J. (2007) Isolation, Characterization and Phylogenetic Analysis of an Acidophilic Heterotrophic Bacterium, Progress in Modern Biomedicine 7(11):1600~1604 (In Chinese)
  3. Zhang Y., He H., Shi W., Wan M., Yang Y. (2006) Freeze-drying Cryoprotectant Optimized by Orthogonal Experiment for Acidithiobacillus ferrooxidans, Progress in Modern Biomedicine 6(5):5~7 (In Chinese)
  4. Yang Y.，Zhang Y.，He H., Wan M.，Shi W., Qiu G. (2006) Optimization of frozen storage cryoprotectant for Acidithiobacillus ferrooxidans by orthogonal experiment, J. Cent. South Univ. (Science and Technology)37(5):891~895 (In Chinese)
  5. Yang Y., Zhang Y., Huang J., Jiang D., Chen Y., Qiu G. (2006) Preservation of Acidithiobacillus ferrooxidans,J. Cent. South Univ. (Science and Technology) 37(3):472~475 (In Chinese)
  6. Yang Y., Shi W., Zhang Y., Xu A., Liu J., Zou L., Qiu G.(2006)Biodesulfurization of coal by Acidithiobacillus ferrooxidans, Ecology and Environmnet 15(2):261~264 (In Chinese)
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