副研究员/高级工程师
王丽贤

王丽贤


  • 职  称:高级工程师
  • 学  历:硕士研究生
  • 通讯地址:天津市空港经济区西七道32号
  • 电  话:022-24828743
  • 邮政编码:300308
  • 电子邮件:wang_lx@tib.cas.cn
  • 部  门:高通量编辑与筛选平台实验室

简历

教育经历

2004.09-2008.06,中国,山东大学,生物技术,学士学位

2008.09-2011.06,中国,中国科学院动物研究所,发育生物学,硕士学位

工作经历

2011.10-2012.04,中国,清华大学生命学院,科研助理

2012.04-至今,中国科学院天津工业生物技术研究所

研究方向

超高通量筛选设备的管理和方法开发。开发了微生物流式筛选固体平板接收器,建立荧光蛋白库、单细胞制样方法、真菌孢子和原生质体筛选体系。

获奖及荣誉

2021 年度、2023 年度“所级优秀”

天工杯”优秀五小创新成果二等奖

代表成果

近三年参与发表论文20余篇。代表论文如下:

1.  Wei Pu,Jinhui Feng,Jiuzhou Chen,Jiao Liu,Xuan Guo,Lixian Wang,Xiaojia Zhao,Ningyun Cai,Wenjuan Zhou,Yu Wang,Ping Zheng and Jibin Sun. Engineering of l-threonine and lproline biosensors by directed evolution of transcriptional regulator SerR and application for high-throughput screening. Bioresources and Bioprocessing. (2025)

2.  Qi Xiao,Jingjing Shi,Lixian Wang,Guoping Zhao,Yanfei Zhang. Coupling genome-wide continuous perturbation with biosensor screening reveals the potential targets in yeast isopentanol synthesis network. Synthetic and Systems Biotechnology.(2025)

3.  Jiao Liu,Xiaojia Zhao,Haijiao Cheng,Yanmei Guo,Xiaomeng Ni, Lixian Wang, Guannan Sun,Xiao Wen,Jiuzhou Chen,Jin Wang,Jingjing An,Xuan Guo,Zhenkun Shi,Haoran Li,Ruoyu Wang,Muqiang Zhao,Xiaoping Liao,Yu Wang,Ping Zheng,Meng Wang,Jibin Sun. Comprehensive screening of industrially relevant components at genome scale using a high-quality gene overexpression collection of Corynebacterium glutamicum. Trends in Biotechnology.(2024)

4.  王丽贤、张玥、夏海容、涂然、王猛 ,微生物流式分选的单细胞样品制备以及存活率提高的方法优化,微生物学通报(2023

5.  Ling Tan,Meizi Liu,Lixian Wang,Guoping Zhao,Yanfei Zhang. Flow cytometry-based high-throughput screening of synthetic peptides for palladium adsorption Journal of Hazardous Materials.(2024)

6.  Yan wang,Shixin Li,Ning Xue,Lixian Wang et al. Modulating sensitivity of erythromycin biosensor for precise high-throughput screening of strains with different characteristics. ACS Synthetic Biology. (2023)

7.  Ningyun Cai,Jiuzhou Chen,Ning Gao,Xiaomeng Ni,Yu Lei,Wei Pu, Lixian Wang. et al. Engineering of the DNA replication and repair machinery to develop binary mutators for rapid genome evolution of Corynebacterium glutamicum. Nucleic Acids Research. (2023)

8.  Jing Chen,Yuxin Zheng,Lixian Wang,Xuefei Pang,Feng Gao,Haixia Xiao,Nairui Huo. Expression,purification,and biological characterization of recombinant human interleukin-31 protein. Biotechnol Appl Biochem.(2023)

9.  Yuxin Zheng,Jing Zhang,Tianling Guo,Jin Cao,Lixian Wang, Jie Zhang,Xuefei Pang,Feng Gao,Hua Sun,Haixia Xiao. Canine interleukin31 binds directly to OSMRβ with higher binding affinity than to IL31RA.3 Biotech. (2023)

10. Jin Qian. et al. Directed evolution of a neutrophilic and mesophilic methanol dehydrogenase based on high-throughput and accurate measurement of formaldehyde. Synthetic and Systems Biotechnology.(2023)(致谢)

11. Pengju Wang. et al. Artificial Diploid Escherichia coli by a CRISPR Chromosome-Doubling Technique.Adv. Sci. (2023)(致谢)

12. Yudan Lu,Xiaomei Zheng,Yu Wang,Lihui Zhang,Lixian Wang, Yu Lei,Tongcun ZhangPing Zheng,Jibin Sun.Evaluation of Aspergillus niger Six Constitutive Strong Promoters by Fluorescent-Auxotrophic Selection Coupled with Flow Cytometry: A Case for Citric Acid Production.Journal of Fungi.(2022)

13. Yuxin Zheng,Zheng Fan,Jing Zhang,Jing Chen,Lixian Wang,Xuefei Pang,Tianling Guo,Jingfang Liu,Feng Gao,Haixia Xiao. Development and characterization of a novel mouse anti-canine oncostatin M receptor beta monoclonal antibody. Biochemical and Biophysical Research Communications 614 (2022)

14. Le Gao,Qi Guo,Ruinan Xu,Haofan Dong,Chichun Zhou,Zhuohang Yu,Zhaokun Zhang,Lixian Wang,Xiaoyi Chen,Xin Wu. Loop optimization of Trichoderma reesei endoglucanases for balancing the activity–stability trade-off through cross-strategy between machine learning and the B-factor analysis.GCB Bioenergy.(2022)

15. Le Gao,Qiuhui Chen,Haofan Dong,Zhaokun Zhang,Tongtong Bao,Lixian Wang,Fan Yang,Xin Wu. Function of four tryptophan residues on Cel7A catalytic efficiency: Insight into cellulase screening strategy based on natural cellulose or cellulose analogs. GCB Bioenergy.(2022)

16. Siwei Li,Jingjing An,Yaqiu Li,Xiagu Zhu,Dongdong Zhao,Lixian Wang,Yonghui Sun,Yuanzhao Yang,Changhao Bi,Xueli Zhang,Meng Wang. Automated high-throughput genome editing platform with an AI learning in situ prediction model. Nature Communications(2022)

17. Wei Pu,Jiuzhou Chen,Pi Liu,Jie Shen,Ningyun Cai,Baoyan Liu,Yu Lei,Lixian Wang,Xiaomeng Ni,Jie Zhang,Jiao Liu,Yingyu Zhou,Wenjuan Zhou,Hongwu Ma,Yu Wang,Ping Zheng,Jibin Sun. Directed evolution of linker helix as an efficient strategy for engineering LysR-type transcriptional regulators as whole-cell biosensors. Biosensors and Bioelectronics.(2022)