李清艳----中国科学院天津工业生物技术研究所

人才队伍

副研究员/高级工程师

李清艳

职　　称：副研究员
学　　历：博士研究生
导师类别：硕士生导师
通讯地址：天津市空港经济区西七道32号
邮政编码：300308
电子邮件：li_qy@tib.cas.cn
部　　门：微生物代谢工程研究组

简历

教育经历：

2006/9–2009/12，南开大学生命科学学院，微生物专业，理学博士

工作经历：

2016/10-2024/10，中国科学院天津工业生物技术研究所，副研究员

2010/1-2016/9，中国科学院天津工业生物技术研究所，助理研究员

2007/9-2009/7，美国明尼苏达大学，土壤、水和大气学院，访问学者

1996/7-2002/9，河南省南召县第一职业高中，专业课教研组，教师

研究方向

主要进行微生物代谢工程和细胞工厂构建及其在类胡萝卜素合成中的应用研究。对萜类化合物合成机制深入研究，主要从能量供应、还原力平衡、限速步骤挖掘等方面开展研究。在能量代谢方面，通过中央代谢模式的调控解决了ATP 和还原力供给与需求的不平衡问题，阐述了ATP和还原力对合成代谢的调控机制。在膜工程方面，通过增加膜结构等确定类胡萝卜素的储存机制；提高微生物细胞工厂合成类胡萝卜素的能力和转化效率，用于生物发酵法合成萜类化合物。

主要成果包括成功构建了酿酒酵母和大肠杆菌底盘细胞，并在此基础上构建了产玫瑰精油、香紫苏醇、人参皂苷、多种类胡萝卜素的细胞工厂，相关研究发表在Metabolic Engineering、Journal of Agricultural and Food Chemistry等期刊。

代表成果

Meng Zhang+, QingYan Li*, Feiyu Fan, Fuping Lu, XueLi Zhang*. Combinatorial metabolic engineering and tolerance evolution of Yarrowia lipolytica for improved γ-decalactone synthesis, Process Biochem. 2023, 132:248-254
Meng Zhang+, Zhongkuo Gong+, Jinlei Tang， Fuping Lu, QingYan Li* and XueLi Zhang*.　Improving astaxanthin production in Escherichia coli by co-utilizing CrtZ enzymes with different substrate preference，Microb Cell Fact. 2022, 21:71
Jie Wang, Dongdong Zhao, Ju Li, Muzi Hu, Xiuqing Xin, Marcus A Price, Qingyan Li, Li Liu, Siwei Li, Susan J Rosser, Chunzhi Zhang, Changhao Bi*, Xueli Zhang*. Helicase-AID:A novel molecular device for base editing at random genomic loci. Metab Eng. 2021,67: 396-402
Zhongkuo Gong+; Honglei Wang*; Jinlei Tang; Qingyan Li*; Xueli Zhang*; Coordinated Expression of Astaxanthin Biosynthesis Genes for Improved Astaxanthin Production in Escherichia coli, J. A gric. Food Chem. 2020, 68(50): 14917-14927
Tao Wu, Siwei Li, Lijun Ye, Dongdong Zhao, Feiyu Fan, Qinyan Li, Bolin Zhang, Changhao Bi*, Xueli Zhang*. Engineering an Artificial Membrane Vesicle Trafficking System (AMVTS) for the Excretion of β-Carotene in Escherichia coli. ACS Synth Biol. 2019. 17;8(5):1037-1046
Jinying Guo, Kunle Hu, Changhao Bi, Qingyan Li*, Xueli Zhang*. Construction of an alternative glycerol-utilization pathway for improved β-carotene production in Escherichia coli. J Ind Microbiol Biotechnol. 2018.45(8):697-705
Qingyan Li, Feiyu Fan, Xiang Gao, Chen Yang, Changhao Bi, Jinlei Tang, Tao Liu, Xueli Zhang*. Balanced activation of IspG and IspH to eliminate MEP intermediate accumulation and improve isoprenoids production in Escherichia coli. Metab Eng. 2017. 44:13-21
Tao Wu, Lijun Ye, Dongdong Zhao, Siwei Li, Qinyan Li, Changhao Bi*, Xueli Zhang*. Membrane engineering - A novel strategy to enhance the production and accumulation of β-carotene in Escherichia coli. Metab Eng. 2017, 43:85-91.
Lijun Ye, Ping He, Qinyan Li, Xueli Zhang*, Changhao Bi*. Type IIs restriction based combinatory modulation technique for metabolic pathway optimization. Microb Cell Fact. 2017, 16:47.
Lijun Ye, Chunzhi Zhang, Changhao Bi, Qinyan Li*, Xueli Zhang*. Combinatory Optimization of Chromosomal Integrated Mevalonate Pathway for β-carotene Production in Escherichia coli. Microb Cell Fact. 2016, 15:202.
Tao Sun, Liangtian Miao, Qinyan Li*, Guanping Dai, Fuping Lu, Tao Liu, Xueli Zhang*, Yanhe Ma. Production of lycopene by metabolically-engineered Escherichia coli. Biotechnol Lett, 2014, 36(7): 1515–1522.
Jing Zhao, Qingyan Li, Tao Sun, Xinna Zhu, Hongtao Xu, Jinlei Tang,Xueli Zhang*, Yanhe Ma. Engineering central metabolic modules of Escherichia coli for improving β-carotene production. Metab Eng. 2013, 17: 42-50.（并列一作）