致力于开发和优化自动化基因编辑与调控工具，并结合基于液滴微流控等高通量筛选技术，建立枯草芽孢杆菌工程菌株自动化铸造平台，从而显著提升菌株设计与研发的效率与通量。以生物活性分子为研究目标，从基因和蛋白水平解析其生物合成途径及调控机制，并通过代谢工程策略提高产量，实现高附加值化学品的绿色生物制造。相关研究成果已发表于 Nat Commun、J. Am. Chem. Soc.、Trends Biotechnol、Metab Eng、Appl Environ Microbiol、ACS Synth Biol、Sci China Life Sci、ChemBioChem等国际期刊。

中国科学院第四届“科苑名匠”（工程菌种铸造科学设施团队）

天津市三八红旗集体（高通量编辑与筛选平台）

山东省研究生优秀科技创新成果二等奖

中国海洋大学第十七届优秀博士论文奖

中国海洋大学优秀毕业生

1. Liu, Y. ^*, Wang, X., Wang, M.* (2026). Resilience-by-design in engineering Bacillus cell factories. Trends in Biotechnology, S0167-7799(26)00049-1.

2. Liu, Y. ^*, Cao, X., Wang, X., Chen, R., Yuan, B., Li, S., Wang, Y., Liao, X., Ni, X., Wang, L., Guo, Y., Ma, H., & Wang, M. * (2026). Multiplex base editing of RBSs rewires Bacillus subtilis metabolism for lycopene overproduction. Metabolic engineering, 93, 169–183.

3. Cao, X., Wang, X., Chen, R., Chen, L., Liu, Y. ^*, &Wang, M.^* (2025). Improving Bacillus subtilis as biological chassis performance by the CRISPR genetic toolkit. ACS synthetic biology, 14(3), 677–688.

4. Wang, Y. ^#*, Cheng, H. ^#, Liu, Y. ^#, Liu, Y., Wen, X., Zhang, K., Ni, X., Gao, N., Fan, L., Zhang, Z., Liu, J., Chen, J., Wang, L., Guo, Y., Zheng, P.^*, Wang, M.^*, Sun, J., & Ma, Y. (2021). In-situ generation of large numbers of genetic combinations for metabolic reprogramming via CRISPR-guided base editing. Nature communications, 12(1), 678.

5. Liu, Y., Cheng, H., Li, H., Zhang, Y., &Wang, M.^* (2023). A Programmable CRISPR/Cas9 Toolkit Improves Lycopene Production in Bacillus subtilis. Applied and environmental microbiology, 89(6), e0023023.

6. Liu, Y., Liu, Y., & Wang, M.^* (2017). Design, Optimization and Application of Small Molecule Biosensor in Metabolic Engineering. Frontiers in microbiology, 8, 2012.

7. Liu, Y. ^#, Liu, Y. ^#, Zheng, P., Wang, Y., & Wang, M.^* (2023). Cytosine Base Editing in Bacteria. Methods in molecular biology, 2606, 219-231.

8. Xiao, F., Dong, S., Liu, Y., Feng, Y., Li, H., Yun, C. H., Cui, Q., & Li, W. ^* (2020). Structural Basis of Specificity for Carboxyl-Terminated Acyl Donors in a Bacterial Acyltransferase. Journal of the American Chemical Society, 142(37), 16031-16038.

9. Liu, Y., Qin, W., Liu, Q., Zhang, J., Li, H., Xu, S., Ren, P., Tian, L., & Li, W. (2016). Genome-wide identification and characterization of macrolide glycosyltransferases from a marine-derived Bacillus strain and their phylogenetic distribution. Environmental microbiology, 18(12), 4770-4781.

10. Qin, W.^#, Liu, Y.^#, Ren, P., Zhang, J., Li, H., Tian, L., & Li, W. (2014). Uncovering a glycosyltransferase provides insights into the glycosylation step during macrolactin and bacillaene biosynthesis. Chembiochem, 15(18), 2747-2753.

11. Liu, Y., Zheng, H., Zhan, G., Qin, W., Tian, L., & Li, W. (2014). Establishment of an efficient transformation protocol and its application in marine-derived Bacillus strain. Science China. Life sciences, 57(6), 627–635.

12. Yu, S. ^#, Price, M. A. ^#, Wang, Y., Liu, Y., Guo, Y., Ni, X., Rosser, S. J., Bi, C., & Wang, M. (2020). CRISPR-dCas9 Mediated Cytosine Deaminase Base Editing in Bacillus subtilis.ACS synthetic biology, 9(7), 1781–1789.

13. Liu, Q. ^#, Ren, P. ^#, Liu, Y., Qin, W., Li, H., & Li, W. (2018). Exploration of the Glycosyltransferase BmmGT1 from a Marine-Derived Bacillus Strain as a Potential Enzyme Tool for Compound Glycol-Diversification.Journal of microbiology and biotechnology, 28(6), 931–937.

14. 李昊霓, 李颖, 于思礼, 涂然，花尔并^*，刘扬^*，王猛^*（2024）. 高通量构建与筛选信号肽库提高枯草芽孢杆菌外源蛋白的表达分泌. 微生物学报, 64(08)，3059-3072.